概述

  • 产品名称
  • 描述
    兔多克隆抗体to PCM1
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Orangutan
  • 免疫原

    A synthetic peptide corresponding to a region between residues 1925 and 1975 of human PCM1.

  • 阳性对照
    • HeLa whole cell lysate; 293T and mouse NIH3T3 cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab72443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 270 kDa.
IP Use a concentration of 2 - 5 µg/ml.

靶标

  • 功能
    Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia.
  • 组织特异性
    Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid.
  • 疾病相关
    Defects in PCM1 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving PCM1 is found in thyroid papillary carcinomas. Translocation t(8;10)(p21.3;q11.2) with RET links the protein kinase domain of RET to the major portion of PCM1.
    Note=A chromosomal aberration involving PCM1 is found in a variety of hematological malignancies including atypical chronic myeloid leukemia (atypical CML) and T-cell lymphoma. Translocation t(8;9)(p22;p24) with JAK2 links the protein kinase domain of JAK2 to the major portion of PCM1.
  • 序列相似性
    Belongs to the PCM1 family.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic granule. Localizes to cytoplasmic granules which are enriched around the centrosome. This centrosomal enrichment requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hPCM-1 antibody
    • PCM-1 antibody
    • pcm1 antibody
    • PCM1_HUMAN antibody
    • Pericentriolar material 1 antibody
    • Pericentriolar material 1 protein antibody
    • PTC4 antibody
    see all

Anti-PCM1 antibody 图像

  • All lanes : Anti-PCM1 antibody (ab72443) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 cell lysate at 50 µg


    Observed band size : 270 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation of PCM1 from 1mg HeLa whole cell lysate by 3 ug ab72443 followed by WB (20% of IP loaded):
    Lane 1: PCM1 precipitate

    Lane 2: Control IgG

    For blotting immunoprecipitated PCM1, ab72443 was used at 1 ug/ml.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-PCM1 antibody (ab72443)参考文献

This product has been referenced in:
  • Schou KB  et al. KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling. Nat Commun 8:14177 (2017). Read more (PubMed: 28134340) »
  • Stephen LA  et al. TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23). Elife 4:N/A (2015). ICC . Read more (PubMed: 26386247) »

See all 4 Publications for this product

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