概述

  • 产品名称Anti-PCK2抗体
    参阅全部 PCK2 一抗
  • 描述
    小鼠单克隆抗体to PCK2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1 - 255 of human PCK2 (AAH01454) with a 26 kDa tag

  • 阳性对照
    • A-431 cell lysate; immunogen

应用

Our Abpromise guarantee covers the use of ab77047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 71 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • 通路Carbohydrate biosynthesis; gluconeogenesis.
  • 疾病相关Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD) [MIM:261650]. A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • 序列相似性Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 4.1.1.32 antibody
    • GTP mitochondrial precursor antibody
    • HGNC:8725 antibody
    • mitochondrial antibody
    • Mitochondrial phosphoenolpyruvate carboxykinase 2 antibody
    • OTTHUMP00000164700 antibody
    • PCK2 antibody
    • PCKGM_HUMAN antibody
    • PE antibody
    • PEP carboxykinase antibody
    • PEPCK antibody
    • PEPCK deficiency mitochondrial antibody
    • PEPCK M antibody
    • PEPCK-M antibody
    • PEPCK2 antibody
    • Phosphoenolpyruvate carboxykinase [GTP] antibody
    • Phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody
    • Phosphoenolpyruvate carboxykinase 2 mitochondrial antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all

Anti-PCK2 antibody 图像

  • Anti-PCK2 antibody (ab77047) at 1/500 dilution + recombinant tagged PCK2 protein (the immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG(H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 71 kDa
    Observed band size : 96 kDa (why is the actual band size different from the predicted?)
  • Anti-PCK2 antibody (ab77047) at 1/500 dilution + A-431 cell lysate at 25 µg

    Secondary
    goat anti-kouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 71 kDa
    Observed band size : 71 kDa

Anti-PCK2 antibody (ab77047)参考文献

This product has been referenced in:
  • Leithner K  et al. PCK2 activation mediates an adaptive response to glucose depletion in lung cancer. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24632615) »
  • Martin SA  et al. Parallel high-throughput RNA interference screens identify PINK1 as a potential therapeutic target for the treatment of DNA mismatch repair-deficient cancers. Cancer Res 71:1836-48 (2011). WB ; Human . Read more (PubMed: 21242281) »

See all 2 Publications for this product

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