概述

  • 产品名称Anti-PCK1抗体
    参阅全部 PCK1 一抗
  • 描述
    兔多克隆抗体to PCK1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Horse, Chicken, Cow, Pig, Zebrafish, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human PCK1.

    (Peptide available as ab87372.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human Liver Rat Liver Mouse Liver

应用

Our Abpromise guarantee covers the use of ab87340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).Can be blocked with PCK1 peptide (ab217264).

靶标

  • 功能Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • 组织特异性Major sites of expression are liver, kidney and adipocytes.
  • 通路Carbohydrate biosynthesis; gluconeogenesis.
  • 疾病相关Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • 序列相似性Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • 翻译后修饰Acetylation is increased on addition of glucose and appears to regulate the protein stability.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cytosolic [GTP] antibody
    • GTP antibody
    • PCK1 antibody
    • PCKGC_HUMAN antibody
    • PEP carboxykinase antibody
    • PEPCK-C antibody
    • PEPCK1 antibody
    • PEPCKC antibody
    • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
    • Phosphoenolpyruvate carboxykinase 1 antibody
    • Phosphoenolpyruvate carboxykinase antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all

Anti-PCK1 antibody 图像

  • All lanes : Anti-PCK1 antibody (ab87340) at 2 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 70 kDa


    Exposure time : 15 minutes

Anti-PCK1 antibody (ab87340)参考文献

ab87340 has not yet been referenced specifically in any publications.

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