概述

  • 产品名称Anti-PCK1抗体
    参阅全部 PCK1 一抗
  • 描述
    山羊多克隆抗体to PCK1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Orangutan
  • 免疫原

    Synthetic peptide:

    C-EKEVEDIEKYLEDQ

    , corresponding to internal sequence (near C terminus) amino acids 586-599 of Human PCK1

  • 阳性对照
    • Human Kidney lysate.

应用

Our Abpromise guarantee covers the use of ab61701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明Peptide ELISA: antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
    • 组织特异性Major sites of expression are liver, kidney and adipocytes.
    • 通路Carbohydrate biosynthesis; gluconeogenesis.
    • 疾病相关Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
    • 序列相似性Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
    • 翻译后修饰Acetylation is increased on addition of glucose and appears to regulate the protein stability.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • cytosolic [GTP] antibody
      • GTP antibody
      • PCK1 antibody
      • PCKGC_HUMAN antibody
      • PEP carboxykinase antibody
      • PEPCK-C antibody
      • PEPCK1 antibody
      • PEPCKC antibody
      • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
      • Phosphoenolpyruvate carboxykinase 1 antibody
      • Phosphoenolpyruvate carboxykinase antibody
      • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
      • Phosphoenolpyruvate carboxykinase, cytosolic antibody
      • Phosphoenolpyruvate carboxylase antibody
      • Phosphopyruvate carboxylase antibody
      see all

    Anti-PCK1 antibody 图像

    • Anti-PCK1 antibody (ab61701) at 0.5 µg/ml + Human Kidney lysate (35µg protein in RIPA buffer)

      Predicted band size : 69 kDa
      Observed band size : 69 kDa

    Anti-PCK1 antibody (ab61701)参考文献

    This product has been referenced in:
    • Jornayvaz FR  et al. Thyroid hormone receptor-a gene knockout mice are protected from diet-induced hepatic insulin resistance. Endocrinology 153:583-91 (2012). WB ; Mouse . Read more (PubMed: 22147010) »

    See 1 Publication for this product

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