概述

  • 产品名称Anti-PCID1抗体
    参阅全部 PCID1 一抗
  • 描述
    兔多克隆抗体to PCID1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: MSVPAFIDIS EEDQAAELRA YLKSKGAEIS EENSEGGLHV DLAQIIEACD, corresponding to N terminal amino acids 1-50 of Human PCID1

  • 阳性对照
    • HepG2 cell lysates and Human kidney tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab69842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2).
  • 组织特异性Broadly expressed.
  • 序列相似性Belongs to the eIF-3 subunit M family.
    Contains 1 PCI domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B5 antibody
    • B5 receptor antibody
    • Dendritic cell protein antibody
    • eIF3m antibody
    • EIF3M_HUMAN antibody
    • Eukaryotic translation initiation factor 3 subunit M antibody
    • Fetal lung protein B5 antibody
    • FLJ29030 antibody
    • GA17 antibody
    • hfl B5 antibody
    • hFL-B5 antibody
    • PCI domain containing 1 (herpesvirus entry mediator) antibody
    • PCI domain-containing protein 1 antibody
    • PCID1 antibody
    • TANGO7 antibody
    see all

Anti-PCID1 antibody 图像

  • Anti-PCID1 antibody (ab69842) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PCID1 with ab69842 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-PCID1 antibody (ab69842)参考文献

This product has been referenced in:
  • Cheshenko N  et al. HSV usurps eukaryotic initiation factor 3 subunit M for viral protein translation: novel prevention target. PLoS One 5:e11829 (2010). WB, ICC/IF ; Human . Read more (PubMed: 20676407) »

See 1 Publication for this product

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