Anti-PCGF1抗体(ab84108)

概述

  • 产品名称Anti-PCGF1抗体
    参阅全部 PCGF1 一抗
  • 描述
    兔多克隆抗体to PCGF1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human PCGF1.

    (Peptide available as ab94828.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: MCF7.

性能

应用

Our Abpromise guarantee covers the use of ab84108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性PCGF1 is a component of the Polycomb group (PcG) multiprotein BCOR complex, a complex required to maintain the transcriptionally repressive state of some genes, such as BCL6 and the cyclin-dependent kinase inhibitor, CDKN1A. It represses CDKN1A expression by binding to its promoter, and this repression is dependent on the retinoic acid response element (RARE element). PCGF1 als promotes cell cycle progression and also enhances cell proliferation, thus it may have a positive role in tumor cell growth.
    • 细胞定位Nuclear
    • 数据库链接
    • 别名
      • PCGF1 antibody
      • 2010002K04Rik antibody
      • Lethal(3)73AH, Drosophila, homolog of antibody
      • MGC10882 antibody
      • Nervous system Polycomb-1 antibody
      • NSPc1 antibody
      • polycomb group ring finger 1 antibody
      • Polycomb group RING finger protein 1 antibody
      • RING finger protein 68 antibody
      • RNF3A-2 antibody
      • RNF68 antibody
      see all

    Anti-PCGF1 antibody 图像

    • Anti-PCGF1 antibody (ab84108) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 29 kDa
      Observed band size : 29 kDa
      Additional bands at : 105 kDa,80 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 20 minutes

    Anti-PCGF1 antibody (ab84108)参考文献

    ab84108 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Oct 19 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"