概述

  • 产品名称Anti-PCDHGB1抗体
    参阅全部 PCDHGB1 一抗
  • 描述
    兔多克隆抗体to PCDHGB1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide, corresponding to N terminal amino acids 181-230 of Human PCDHGB1

  • 阳性对照
    • Fetal lung lysate.

性能

应用

Our Abpromise guarantee covers the use of ab81072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/62500.

靶标

  • 相关性The PCDHGB1 gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
  • 细胞定位Cell membrane; Single-pass type I membrane protein.
  • 数据库链接
  • 别名
    • MGC119466 antibody
    • MGC119467 antibody
    • MGC119469 antibody
    • PCDH gamma B1 antibody
    • Protocadherin gamma B1 antibody
    • Protocadherin gamma B1 precursor antibody
    • Protocadherin gamma subfamily B 1 antibody
    see all

Anti-PCDHGB1 antibody 图像

  • Anti-PCDHGB1 antibody (ab81072) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 85 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

Anti-PCDHGB1 antibody (ab81072)参考文献

ab81072 has not yet been referenced specifically in any publications.

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