The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml.
Use a concentration of 3 µg/ml.
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
相关性PCDH8 (Protocadherin 8) belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. It is thought to function in cell-adhesion in a calcium-dependent manner. It is predominantly expressed in brain, localizing to synaptic junctions.
细胞定位Cell Membrane. Single-pass type I membrane protein.
PCDH8 antibody (ab55507) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
Western blot - PCDH8 antibody (ab55507)
PCDH8 antibody (ab55507) at 1ug/lane + COLO cell lysate at 25ug/lane.
Flow Cytometry-Anti-PCDH8 antibody(ab55507)
Overlay histogram showing SH-SY5Y cells stained with ab55507 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55507, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Anti-PCDH8 antibody (ab55507)参考文献
has not yet been referenced specifically in any publications.