Anti-PCDH7抗体(ab55506)

概述

  • 产品名称Anti-PCDH7抗体
    参阅全部 PCDH7 一抗
  • 描述
    小鼠单克隆抗体to PCDH7
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Xenopus laevis, Recombinant Fragment
    预测可用于: Human
  • 免疫原

    Recombinant fragment: KQLLRYRLAE EGPADVRIGN VASDLGIVTG SGEVTFSLES GSEYLKIDNL TGELSTSERR IDREKLPQCQ MIFDENECFL DFEVSVIGPS QSWV, corresponding to amino acids 31-125 of Human PCDH7

性能

应用

Our Abpromise guarantee covers the use of ab55506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 组织特异性Expressed predominantly in brain and heart and at lower levels in various other tissues.
    • 序列相似性Contains 7 cadherin domains.
    • 翻译后修饰Isoform C is phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BH Pcdh antibody
      • BH protocadherin antibody
      • BH-Pcdh antibody
      • BHPCDH antibody
      • Brain heart protocadherin antibody
      • Brain-heart protocadherin antibody
      • PCDH 7 antibody
      • PCDH7 antibody
      • PCDH7_HUMAN antibody
      • Protocadherin 7 antibody
      • Protocadherin-7 antibody
      see all

    Anti-PCDH7 antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab55506 PCDH7 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa

    Anti-PCDH7 antibody (ab55506)参考文献

    ab55506 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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    Thank you for your reply and the additional information.
    I have one last question:
    Since a truncated PCDH7 construct (+ GFP) was used, I assume the construct contained the antibody binding site (immunogen: KQLLRYRLAE EGPADVRIGN VASDLGIVTG SGE...

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    Thank you for sending the protocol details. This very helpful.
    I have a few additional questions:
    1) At what MW was the band for GFP-PCDH7 seen?
    2) Does the secondary antibody work well with other primaries?
    3) What lysis buffer was...

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    Thank you for contacting us.
    I am sorry to hear the antibody is not working as expected.
    As we discussed over the phone, can you please send me the WB image as well as protocol information you have from your colleagues, so that we can evaluat...

    Read More

    Thank you for your call today.

    As we discussed, please let me know which of these options you would prefer, and I'll be happy to help you further.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Xenopus laevis Tissue lysate - whole (Eye)
    Loading amount 15 µg
    Specification Eye
    Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 18 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"