Anti-PCDH19抗体(ab57510)

概述

  • 产品名称Anti-PCDH19抗体
    参阅全部 PCDH19 一抗
  • 描述
    小鼠单克隆抗体to PCDH19
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: RLVPRDVEET DKMNVVSCSS LTSSLNYFDY HQQTLPLGCR RSESTFLNVE NQNTRNTSAN HIYHHSFNSQ GPQQPDLIIN GAPLPETENY SFDSNYVNSR , corresponding to amino acids 241-341 of Human PCDH19

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab57510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Potential calcium-dependent cell-adhesion protein.
  • 组织特异性Moderately expressed in all regions of the brain examined, with lowest levels found in the cerebellum. Moderate expression is also found in ovary, and low expression in all other tissues tested. Also detected in primary skin fibroblast.
  • 疾病相关Epileptic encephalopathy, early infantile, 9
  • 序列相似性Contains 6 cadherin domains.
  • 发展阶段Expressed in developing cortical plate, amygdala and subcortical regions and in the ganglionic eminence.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA1313 antibody
    • PCD19_HUMAN antibody
    • PCDH19 antibody
    • Protocadherin 19 antibody
    • Protocadherin-19 antibody
    see all

Anti-PCDH19 antibody 图像

  • Western blot against tagged recombinant protein immunogen using ab57510 PCDH19 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab57510 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57510, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SH-SY5Y cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-PCDH19 antibody (ab57510)参考文献

ab57510 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (postnatal day 10 cerebral cortex)
Gel Running Conditions Reduced Denaturing
Loading amount 100 µg
Specification postnatal day 10 cerebral cortex
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Apr 17 2015

Application Immunocytochemistry
Sample Human Cultured Cells (Human PCDH19 transfected in HEK 293FT cell line)
Specification Human PCDH19 transfected in HEK 293FT cell line
Permeabilization Yes - permeabilization was achieved with GDB buffer during ab incubation
Fixative Paraformaldehyde
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提交于 Mar 11 2015

Application Western blot
Loading amount 2 µg
Gel Running Conditions Reduced Denaturing (7,5% gel)
Sample Human Cell lysate - whole cell (Human PCDH19 transfected in HEK 293FT cell line)
Specification Human PCDH19 transfected in HEK 293FT cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Mar 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"