Anti-PCCB抗体(ab70416)

概述

  • 产品名称Anti-PCCB抗体
    参阅全部 PCCB 一抗
  • 描述
    小鼠多克隆抗体to PCCB
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human PCCB protein (NP_000523.2).

  • 阳性对照
    • PCCB transfected 293T lysate and human liver tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab70416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 通路Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3.
  • 疾病相关Defects in PCCB are the cause of propionic acidemia type II (PA-2) [MIM:606054]. PA-2 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein.
  • 序列相似性Belongs to the AccD/PCCB family.
    Contains 1 carboxyltransferase domain.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp451E113 antibody
    • PCCase subunit beta antibody
    • pccB antibody
    • PCCB_HUMAN antibody
    • pccBC Complementation group antibody
    • Propanoyl CoA:carbon dioxide ligase subunit beta antibody
    • Propanoyl-CoA:carbon dioxide ligase subunit beta antibody
    • Propionyl CoA carboxylase beta chain, mitochondrial antibody
    • propionyl Coenzyme A carboxylase, beta polypeptide antibody
    • Propionyl-CoA carboxylase , beta subunit antibody
    • Propionyl-CoA carboxylase beta chain, mitochondrial antibody
    • R74805 antibody
    see all

Anti-PCCB antibody 图像

  • Anti-PCCB antibody (ab70416) at 1/500 dilution + human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PCCB antibody (ab70416) at 1/500 dilution

    Lane 1 : PCCB transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab70416 staining in normal human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PCCB antibody (ab70416)参考文献

ab70416 has not yet been referenced specifically in any publications.

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