概述

  • 产品名称Anti-PCCA抗体
    参阅全部 PCCA 一抗
  • 描述
    鸡多克隆抗体to PCCA
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant fragment:

    IRGVHNIALLREVIINSRFVKGDISKFLSDVYPDGFKGHMLTKSEKNQLL AIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASN

    , corresponding to amino acids 450-703 of Human PCCA.

性能

应用

Our Abpromise guarantee covers the use of ab16307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution. PubMed: 19699272
WB 1/2000. Predicted molecular weight: 78 kDa.

靶标

  • 通路Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3.
  • 疾病相关Defects in PCCA are the cause of propionic acidemia type I (PA-1) [MIM:606054]. PA-1 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein.
  • 序列相似性Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • mitochondrial antibody
    • Pcca antibody
    • pccA COMPLEMENTATION GROUP antibody
    • PCCA_HUMAN antibody
    • PCCase subunit alpha antibody
    • Propanoyl CoA:carbon dioxide ligase subunit alpha antibody
    • Propanoyl-CoA:carbon dioxide ligase subunit alpha antibody
    • Propionyl CoA carboxylase alpha chain antibody
    • Propionyl CoA carboxylase alpha chain mitochondrial antibody
    • Propionyl CoA carboxylase alpha polypeptide antibody
    • Propionyl coenzyme A carboxylase alpha polypeptide antibody
    • Propionyl-CoA carboxylase alpha chain antibody
    see all

Anti-PCCA antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult rat brain labelling PCCA with ab16307. A - cortex. (B) - hippocampus, (C) - caudate putamen. Bar: 100 μm.

Anti-PCCA antibody (ab16307)参考文献

This product has been referenced in:
  • Ballhausen D  et al. Evidence for catabolic pathway of propionate metabolism in CNS: expression pattern of methylmalonyl-CoA mutase and propionyl-CoA carboxylase alpha-subunit in developing and adult rat brain. Neuroscience 164:578-87 (2009). IHC-P ; Rat . Read more (PubMed: 19699272) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Heart)
Loading amount 50 µg
Specification Heart
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Dec 01 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (bone marrow derived-macrophages)
Loading amount 30 µg
Specification bone marrow derived-macrophages
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 01 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"