概述

  • 产品名称Anti-PCBP1抗体
    参阅全部 PCBP1 一抗
  • 描述
    兔多克隆抗体to PCBP1
  • 经测试应用适用于: IHC-P, ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A synthetic peptide corresponding to a region of Human PCBP1

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab74793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 mg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.
  • 组织特异性Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
  • 序列相似性Contains 3 KH domains.
  • 翻译后修饰Phosphorylated; lowers poly(rC)-binding activity.
  • 细胞定位Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha-CP1 antibody
    • Heterogeneous nuclear ribonucleoprotein E1 antibody
    • heterogenous nuclear ribonucleoprotein E1 antibody
    • heterogenous nuclear ribonucleoprotein X antibody
    • hnRNP E1 antibody
    • hnRNP-E1 antibody
    • hnRNP-X antibody
    • HNRPE1 antibody
    • HNRPX antibody
    • nucleic acid binding protein sub 2.3 antibody
    • Nucleic acid- binding protein SUB2.3 antibody
    • Nucleic acid-binding protein SUB2.3 antibody
    • PCBP1 antibody
    • PCBP1_HUMAN antibody
    • poly(rC) binding protein 1 antibody
    • Poly(rC)-binding protein 1 antibody
    • rCbinding protein 1 antibody
    see all

Anti-PCBP1 antibody 图像



  • Predicted band size : 37 kDa
    Western blot analysis of Jurkat cell lysate labeling PCBP1 with ab74793 at 1.0µg/ml.


  • Predicted band size : 37 kDa
    Western Blot using ab74793 at 1.0ug/ml. Sample type: Human MCF7 Lysate
  • ab74793 staining PCBP1 in Human breast Tissue by Immunohistochemistry (Paraffin-embedded sections). Primary antibody used at 5 μg/ml

  • ab74793 staining PCBP1 in Human spleen Tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) after heat treatment antigen retrieval. Primary antibody used at 5 μg/ml

  • Anti-PCBP1 antibody (ab74793) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
  • ICC/IF image of ab74793 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74793, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab74793 at 5µg/ml staining PCBP1 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue).
  • All lanes : Anti-PCBP1 antibody (ab74793) at 1 µg/ml

    Lane 1 : Whole cell lysate prepared from MEF cells
    Lane 2 : Whole cell lysate prepared from F9 cells
    Lane 3 : Whole cell lysate prepared from GC1 cells
    Lane 4 : Whole cell lysate prepared from NIH-3T3 cells

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP conjugated pig anti-rabbit polyclonal
    Developed using the ECL technique

    Predicted band size : 37 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

Anti-PCBP1 antibody (ab74793)参考文献

This product has been referenced in:
  • Ho JJ  et al. Active stabilization of human endothelial nitric oxide synthase mRNA by hnRNP E1 protects against antisense RNA and microRNAs. Mol Cell Biol 33:2029-46 (2013). Human . Read more (PubMed: 23478261) »
  • Sutherland JM  et al. Suppressor of cytokine signaling 4 (SOCS4): moderator of ovarian primordial follicle activation. J Cell Physiol 227:1188-98 (2012). WB, IHC-P ; Mouse . Read more (PubMed: 21604262) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse cell lines)
Loading amount 30 µg
Specification Mouse cell lines
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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Verified customer

提交于 Feb 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human cancer cell lines)
Loading amount 20 µg
Specification Human cancer cell lines
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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Abcam user community

Verified customer

提交于 Feb 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 30 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Nov 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"