概述

  • 产品名称Anti-PCB抗体
    参阅全部 PCB 一抗
  • 描述
    山羊多克隆抗体to PCB
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Horse, Cow, Dog, Human, Pig, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    C-KFKEVKKAYVEANQ

    , corresponding to internal sequence amino acids 886-899 of Human PCB (NP_000911.2; NP_001035806.1; NP_071504.2).

  • 阳性对照
    • Mouse Liver lysate.
  • 常规说明All reported variants (NP_000911.2; NP_001035806.1; NP_071504.2) represent identical protein.

性能

应用

Our Abpromise guarantee covers the use of ab77376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明Peptide ELISA: Antibody detection limit dilution 1/16000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
    • 通路Carbohydrate biosynthesis; gluconeogenesis.
    • 疾病相关Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
    • 序列相似性Contains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • 细胞定位Mitochondrion matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • mitochondrial antibody
      • OTTHUMP00000235155 antibody
      • OTTHUMP00000235156 antibody
      • PC antibody
      • PCB antibody
      • Pcx antibody
      • PYC_HUMAN antibody
      • Pyruvate carboxylase antibody
      • Pyruvate carboxylase mitochondrial antibody
      • Pyruvic carboxylase antibody
      see all

    Anti-PCB antibody 图像

    Anti-PCB antibody (ab77376)参考文献

    This product has been referenced in:
    • Jornayvaz FR  et al. Thyroid hormone receptor-a gene knockout mice are protected from diet-induced hepatic insulin resistance. Endocrinology 153:583-91 (2012). WB ; Mouse . Read more (PubMed: 22147010) »
    • Jornayvaz FR  et al. A high-fat, ketogenic diet causes hepatic insulin resistance in mice, despite increasing energy expenditure and preventing weight gain. Am J Physiol Endocrinol Metab 299:E808-15 (2010). WB ; Mouse . Read more (PubMed: 20807839) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"