概述
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产品名称Anti-PCB抗体
参阅全部 PCB 一抗 -
描述山羊多克隆抗体to PCB
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经测试应用适用于: WB, ELISA, IHC-Pmore details
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种属反应性与反应: Mouse, Human
预测可用于: Rat, Cow, Dog, Pig
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免疫原
Synthetic peptide:
KFKEVKKAYVEANQ
with a Cysteine residue linker, corresponding to internal sequence amino acids 886-899 of Human PCB (NP_000911.2). -
阳性对照
- Human liver tissue; Mouse liver lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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存储溶液pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度Protein G purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab115579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.03 - 1 µg/ml. Predicted molecular weight: 130 kDa. | |
| ELISA | 1/16000. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
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通路Carbohydrate biosynthesis; gluconeogenesis.
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疾病相关Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
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序列相似性Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
细胞定位Mitochondrion matrix.
- Information by UniProt
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数据库链接
- Entrez Gene: 338471 Cow
- Entrez Gene: 5091 Human
- Entrez Gene: 18563 Mouse
- Entrez Gene: 25104 Rat
- Omim: 608786 Human
- SwissProt: Q29RK2 Cow
- SwissProt: P11498 Human
- SwissProt: Q05920 Mouse
see all -
别名
- mitochondrial antibody
- OTTHUMP00000235155 antibody
- OTTHUMP00000235156 antibody
see all
Anti-PCB antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCB antibody (ab115579)ab115579, at 5µg/ml, staining PCB in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen. -
Western blot - Anti-PCB antibody (ab115579)Anti-PCB antibody (ab115579) at 0.03 µg/ml + Mouse Liver lysate in RIPA buffer at 35 µg
Developed using the ECL technique
Predicted band size : 130 kDa
Anti-PCB antibody (ab115579)参考文献
ab115579 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"