Anti-PCB抗体[3H2AD9] (ab110314)

概述

  • 产品名称Anti-PCB抗体[3H2AD9]
    参阅全部 PCB 一抗
  • 描述
    小鼠单克隆抗体[3H2AD9] to PCB
  • 经测试应用适用于: ICC/IF, Flow Cyt, IP, In-Cell ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Purified pyruvate carboxylase from bovine liver

  • 阳性对照
    • Human fibroblast and HeLa cells; Human liver tissue lysate.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml. For 2 hours.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 5 µg/ml.

靶标

  • 功能Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
  • 通路Carbohydrate biosynthesis; gluconeogenesis.
  • 疾病相关Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
  • 序列相似性Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
    Contains 1 carboxyltransferase domain.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • mitochondrial antibody
    • OTTHUMP00000235155 antibody
    • OTTHUMP00000235156 antibody
    • PC antibody
    • PCB antibody
    • Pcx antibody
    • PYC_HUMAN antibody
    • Pyruvate carboxylase antibody
    • Pyruvate carboxylase mitochondrial antibody
    • Pyruvic carboxylase antibody
    see all

Anti-PCB antibody [3H2AD9] 图像

  • Upper image shows: Immunocytochemistry analysis using ab110314 at 5µg/ml staining PCB in Human fibroblast cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
    Note: The target protein locates to the mitochondrial matrix.
    Lower image shows cells co-stained with an antibody against PDH (green), an enzyme also located in the mitochondrial matrix. The composite image shows an identical mitochondrial pattern for both antibodies indicated by merged orange color.
  • Detection of ab110314 by immunoprecipitation staining of 130kDa PCB in human liver lysate.
  • Flow cytometric analysis using ab110314 at 1µg/ml staining PCB in HeLa cells (blue). Isotype control antibody (red).

Anti-PCB antibody [3H2AD9] (ab110314)参考文献

ab110314 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"