Anti-PCB抗体[10G7BF7] (ab123451)

概述

  • 产品名称Anti-PCB抗体[10G7BF7]
    参阅全部 PCB 一抗
  • 描述
    小鼠单克隆抗体[10G7BF7] to PCB
  • 经测试应用适用于: In-Cell ELISA, WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Purified PCB from Cow liver.

  • 阳性对照
    • HepG2, Rat H9C2, Rat H4IIE, Mouse MEF cell lysates; Purified Cow liver PCB; HDFn cells.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab123451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
In-Cell ELISA Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 130 kDa.
ICC Use a concentration of 1 µg/ml.

靶标

  • 功能Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
  • 通路Carbohydrate biosynthesis; gluconeogenesis.
  • 疾病相关Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
  • 序列相似性Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
    Contains 1 carboxyltransferase domain.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • mitochondrial antibody
    • OTTHUMP00000235155 antibody
    • OTTHUMP00000235156 antibody
    • PC antibody
    • PCB antibody
    • Pcx antibody
    • PYC_HUMAN antibody
    • Pyruvate carboxylase antibody
    • Pyruvate carboxylase mitochondrial antibody
    • Pyruvic carboxylase antibody
    see all

Anti-PCB antibody [10G7BF7] 图像

  • All lanes : Anti-PCB antibody [10G7BF7] (ab123451) at 1 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : Hela
    Lane 3 : H9C2
    Lane 4 : MEF
    Lane 5 : Recombinant PC

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat-anti-Mouse HRP at 1/3000 dilution

    Predicted band size : 130 kDa
  • ab123451 at 1µg/ml staining PCB in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).

Anti-PCB antibody [10G7BF7] (ab123451)参考文献

ab123451 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"