概述

  • 产品名称Anti-PC4抗体
    参阅全部 PC4 一抗
  • 描述
    兔多克隆抗体to PC4
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 25 and 75 of human PC4, NP_006704.2.

  • 阳性对照
    • Whole cell lysate from Hela cells.

性能

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应用

Our Abpromise guarantee covers the use of ab72132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 18, 190 kDa (predicted molecular weight: 14 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
  • 序列相似性Belongs to the transcriptional coactivator PC4 family.
  • 翻译后修饰Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activated RNA polymerase II transcriptional coactivator p15 antibody
    • Interferon related developmental regulator 1 antibody
    • MGC102747 antibody
    • p14 antibody
    • P15 antibody
    • PC4 antibody
    • PC4 LSB antibody
    • Positive cofactor 4 antibody
    • RPO2TC1 antibody
    • Sub1 antibody
    • SUB1 homolog antibody
    • TCP4_HUMAN antibody
    see all

Anti-PC4 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PC4 with ab72132 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PC4 antibody (ab72132) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg


    Predicted band size : 14 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 190 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation/ Western Blot of PC4.
    Lane 1: ab72132 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.

    ab72132 at 0.1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection:Chemiluminescence with an exposure time of 30 seconds.
  • IHC image of ab72132 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72132, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PC4 antibody (ab72132)参考文献

ab72132 has not yet been referenced specifically in any publications.

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