概述

  • 产品名称Anti-PC4抗体
    参阅全部 PC4 一抗
  • 描述
    兔多克隆抗体to PC4
  • 特异性ab23594 reacts with PC4 (activated RNA polymerase II transcription cofactor 4).
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 73 - 127 of Human PC4.

  • 阳性对照
    • HepG2 cell lysate for WB Human Liver for IHC-p

性能

应用

Our Abpromise guarantee covers the use of ab23594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
  • 序列相似性Belongs to the transcriptional coactivator PC4 family.
  • 翻译后修饰Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activated RNA polymerase II transcriptional coactivator p15 antibody
    • Interferon related developmental regulator 1 antibody
    • MGC102747 antibody
    • p14 antibody
    • P15 antibody
    • PC4 antibody
    • PC4 LSB antibody
    • Positive cofactor 4 antibody
    • RPO2TC1 antibody
    • Sub1 antibody
    • SUB1 homolog antibody
    • TCP4_HUMAN antibody
    see all

Anti-PC4 antibody 图像

  • Anti-PC4 antibody (ab23594) at 2 µg/ml + HepG2 cell lysate.

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling PC4 with ab23594 at a concentration of 4-8µg/ml. Positive cells marked by arrows are hepatocytes. Magnification 400X.

Anti-PC4 antibody (ab23594)参考文献

ab23594 has not yet been referenced specifically in any publications.

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