概述

  • 产品名称Anti-PC1/3抗体
    参阅全部 PC1/3 一抗
  • 描述
    兔多克隆抗体to PC1/3
  • 经测试应用适用于: IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse PC1/3 aa 111-127.
    Sequence:

    SVQKDSALDLFNDPMWN


    (Peptide available as ab5011)

  • 阳性对照
    • WB: mouse pituitary extract IHC-P: FFPE mouse small intestine tissue sections.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab3532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 80 kDa).Can be blocked with PC1/3 peptide (ab5011).

靶标

  • 功能Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.
  • 疾病相关Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.
  • 序列相似性Belongs to the peptidase S8 family. Furin subfamily.
  • 细胞定位Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BDP antibody
    • NEC 1 antibody
    • NEC1 antibody
    • NEC1_HUMAN antibody
    • Neuroendocrine convertase 1 antibody
    • PC1 antibody
    • PC3 antibody
    • PCSK1 antibody
    • Prohormone convertase 1 antibody
    • Prohormone convertase 1/3 antibody
    • Prohormone convertase 3 antibody
    • Proprotein convertase 1 antibody
    • SPC3 antibody
    see all

Anti-PC1/3 antibody 图像

  • IHC image of PC1/3 staining in Mouse small intestine formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3532, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DAB. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



  • Predicted band size : 80 kDa
    Western blot detection of PC1/3 from mouse pituitary extract using ab3532.
  • Anti-PC1/3 antibody (ab3532) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 87 + 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
    PC1/3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The band observed at 68 kDa could potentially be a cleaved form of PC1/3 due to the presence of a 27 amino acid signal peptide and an 83 amino acid propeptide.

Anti-PC1/3 antibody (ab3532)参考文献

This product has been referenced in:
  • Irwin N  et al. Chemical cholecystokinin receptor activation protects against obesity-diabetes in high fat fed mice and has sustainable beneficial effects in genetic ob/ob mice. Biochem Pharmacol : (2012). Read more (PubMed: 23085436) »
  • Gagnon J  et al. Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine. Regul Pept 152:54-60 (2009). Read more (PubMed: 18706454) »

See all 2 Publications for this product

Product Wall

I have received the following information from the scientist who tested ab3532: "The alignment of the C-terminal peptides shows non cross reactivity to mouse is not surprising since there are not 5 contiguous amino acids that are identical in both seq...

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Thank you very much for your protocol details and your order number. I firstly looked at your protocol and think it is very good. I found references for the presence of both PC1 and PC2 in STC-1 cells (Yoon et al, Endocrinol. 1997 for example) and the...

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