This PC-12 extract (Hypoxia) was tested positively in Western blot and EMSA for NFKB p50 and Pax-3.
Before harvesting the cells were deprived of oxygen for 4 hours.
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Generated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.
has not yet been referenced specifically in any publications.