PC12核extract裂解液(forskolin stimulated) (ab14885)

概述

  • 产品名称
    PC12核extract裂解液(forskolin stimulated)
    参阅全部 PC12 细胞裂解液
  • 描述
    PC12核extract裂解液
  • 常规说明
    To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC. The protein content has been determined by a Bradford-based assay. The PC12 nuclear extracts have been collected after a 30-minute incubation with or without forskolin (20 µM).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.50
    Constituents: 0.48% HEPES, 2.34% Sodium chloride, 20% Glycerol, 0.003% EDTA, 0.042% Sodium fluoride, 0.0002% Sodium molybdate, 0.018% Sodium orthovanadate, 0.216% Beta glycerophosphate, 0.015% DTT, 0.37% PBS
    Note: With Protease inhibitor
  • Concentration information loading...
  • 研究领域
  • 背景
    Generated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.

文献

ab14885 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your enquiry. Forskolin is a compound that activates the cAMP/PKA-dependent signaling pathway; it activates adenylate cyclase and hence elevates intracellular levels of the second messenger cAMP. cAMP controls metabolism, the cytoskel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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