PC12核extract裂解液(ab14884)

概述

  • 产品名称PC12核extract裂解液
    参阅全部 PC12 细胞裂解液
  • 常规说明Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

性能

  • 背景Generated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.

PC12 nuclear extract lysate (ab14884)参考文献

ab14884 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

The product ab29545 is a whole cell lysate not a nuclear lysate. The only nuclear lysate we have in stock is the ab14655that you currently have.

If you still wish for me to send you ab29545, the whole cell ...

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Thank you for that information.

I do not think adding 4ul more will increase your signal and since you can only load a maximum of 22ul/well, I would like to send you ab29545 as a free of charge replacement. This HeLa cell lysate is already in...

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Thank you for your reply.

I appreciate your patience in this matter, but when you say you loaded 2.5mg/ml of total protein, how many microlitres of lysate is that? I ask, as we send 100ug of total protein at at concentration of 2.5mg/ml (ther...

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Thank you for contacting Abcam.

I am sorry about the problems you have been having with ab14655, HeLa cell lysate. I was wondering if you could tell me how much total protein you loaded of the cell lysate. I ask as there does seem to be a sig...

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