The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 22011226
Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
PBX2 is a ubiquitously expressed member of the TALE/PBX homeobox family and was identified by its similarity to a homeobox gene involved in
t(1;19) translocation in acute pre-B-cell leukemias. PBX2 is a transcriptional activator which binds to the TLX1 promoter, binding to the sequence 5'-ATCAATCAA-3'. It forms heterodimers with MEIS1, involved in activation of PF4 transcription, and heterotrimers with MEIS1 and HOXA9. The gene is located within the major histocompatibility complex (MHC) on chromosome 6.
Chatterjee S et al. Conserved and non-conserved enhancers direct tissue specific transcription in ancient germ layer specific developmental control genes. BMC Dev Biol11:63 (2011).
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