概述

  • 产品名称
  • 描述
    山羊多克隆抗体to PBR
  • 宿主
    Goat
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Peptide with sequence C-

    RDNSGRRGGSRLPE

    , corresponding to aa 156-169 from the C Terminus of mouse PBR (NP_033905.3)

  • 阳性对照
    • Mouse Spleen lysate

性能

应用

Our Abpromise guarantee covers the use of ab92291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).

靶标

  • 功能
    Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells.
  • 组织特异性
    Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.
  • 序列相似性
    Belongs to the TspO/BZRP family.
  • 细胞定位
    Mitochondrion membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Benzodiazapine receptor (peripheral) antibody
    • Benzodiazepine peripheral binding site antibody
    • BPBS antibody
    • BZRP antibody
    • DBI antibody
    • IBP antibody
    • Isoquinoline carboxamide-binding protein antibody
    • MBR antibody
    • mDRC antibody
    • Mitochondrial benzodiazepine receptor antibody
    • PBR antibody
    • PBS antibody
    • Peripheral benzodiazepine receptor antibody
    • Peripheral benzodiazepine receptor-related protein antibody
    • Peripheral type benzodiazepine receptor antibody
    • Peripheral-type benzodiazepine receptor antibody
    • pk18 antibody
    • PKBS antibody
    • PTBR antibody
    • Ptbzr antibody
    • PTBZR02 antibody
    • RATPTBZR02 antibody
    • translocator protein (18kDa) antibody
    • Translocator protein antibody
    • Tspo antibody
    • Tspo1 antibody
    • TSPOA_HUMAN antibody
    see all

图片

  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells staining PBR. Cells were permeabilized with 0.15% Triton. Samples were incubated with primary anitbody for 1 hour at 10µg/ml. An Alexa Fluor 488 was used as the secondary antibody. DAPI was used as a nuclear counterstain. Unimmunized goat IgG (10µg/ml) was used as the negative control.
  • All lanes :

    Lane 1 : Mouse Kidney lysate
    Lane 2 : Rat Adrenal Gland lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 16 kDa

  • Immunohistochemical analysis of human adipose tissue labeling PBR. Steamed antigen retrieval with citrate buffer pH
  • Immunohistochemical analysis of human squamous epithelium labeling PBR. Steamed antigen retrieval with citrate buffer pH
  • Immunohistochemical analysis of human renal tubules labeling PBR. Steamed antigen retrieval with citrate buffer pH 6.
  • All lanes : Anti-PBR antibody (ab92291) at 1 µg/ml

    Lane 1 : Mouse Kidney Lysate in RIPA buffer
    Lane 2 : Mouse Spleen Lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 16 kDa

  • Anti-PBR antibody (ab92291) at 1 µg/ml + Mouse Spleen lysate in RIPA buffer at 35 µg

    Predicted band size: 16 kDa
    Observed band size: 18 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab92291 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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