概述

  • 产品名称Anti-PBR抗体
    参阅全部 PBR 一抗
  • 描述
    兔多克隆抗体to PBR
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Horse, Dog, Human, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse PBR.

  • 阳性对照
    • This antibody gave a positive signal in Mouse Brown Adipose tissue.

性能

应用

Our Abpromise guarantee covers the use of ab108489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 19 kDa).

靶标

  • 功能Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells.
  • 组织特异性Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.
  • 序列相似性Belongs to the TspO/BZRP family.
  • 细胞定位Mitochondrion membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Benzodiazapine receptor (peripheral) antibody
    • Benzodiazepine peripheral binding site antibody
    • BPBS antibody
    • BZRP antibody
    • DBI antibody
    • IBP antibody
    • Isoquinoline carboxamide-binding protein antibody
    • MBR antibody
    • mDRC antibody
    • Mitochondrial benzodiazepine receptor antibody
    • PBR antibody
    • PBS antibody
    • Peripheral benzodiazepine receptor antibody
    • Peripheral benzodiazepine receptor-related protein antibody
    • Peripheral type benzodiazepine receptor antibody
    • Peripheral-type benzodiazepine receptor antibody
    • pk18 antibody
    • PKBS antibody
    • PTBR antibody
    • Ptbzr antibody
    • PTBZR02 antibody
    • RATPTBZR02 antibody
    • translocator protein (18kDa) antibody
    • Translocator protein antibody
    • Tspo antibody
    • Tspo1 antibody
    • TSPOA_HUMAN antibody
    see all

Anti-PBR antibody 图像

  • Anti-PBR antibody (ab108489) at 1 µg/ml + P0 Mouse Brown Adipose Tissue Mouse Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

Anti-PBR antibody (ab108489)参考文献

ab108489 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (H9C2, cardiomyoblast cell line)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 20 µg
Specification H9C2, cardiomyoblast cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 10 2016

Abreviews
Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - Tween 20
Sample Horse Cell (bone marrow derived mesenchymal stromal cells)
Specification bone marrow derived mesenchymal stromal cells
Gating Strategy 7-AAD negative cells beside debris
Preparation Cell harvesting/tissue preparation method: By Trypsin detached and wasched plastic adherent cells from culture
Sample buffer: PBS+BSA+NaN3+goat serum+Tween20
Username

Ms. Carmen Schröck

Verified customer

提交于 Aug 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"