重组
RabMAb

Anti-PBP抗体[EPR2875Y] (ab76582)

概述

  • 产品名称
    Anti-PBP抗体[EPR2875Y]
    参阅全部 PBP 一抗
  • 描述
    兔单克隆抗体[EPR2875Y] to PBP
  • 经测试应用
    适用于: Flow Cyt, IHC-P, IHC-Fr, WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • WB: HeLa, A549, HepG2 and A431 cell lysate. IHC-P: Human brain tissue. Flow Cyt: HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-PBP antibody (HRP) [EPR2875Y] (ab202955)

性能

应用

Our Abpromise guarantee covers the use of ab76582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/200.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 22542739
WB 1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IP 1/10.
ICC/IF 1/100.

靶标

  • 功能
    Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
    HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
  • 序列相似性
    Belongs to the phosphatidylethanolamine-binding protein family.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Epididymis luminal protein 210 antibody
    • Epididymis secretory protein Li 34 antibody
    • Epididymis secretory protein Li 96 antibody
    • HCNP antibody
    • HCNPpp antibody
    • HEL 210 antibody
    • HEL S 34 antibody
    • HEL S 96 antibody
    • Hippocampal cholinergic neurostimulating peptide antibody
    • Neuropolypeptide h3 antibody
    • PBP antibody
    • PEBP antibody
    • PEBP 1 antibody
    • PEBP-1 antibody
    • Pebp1 antibody
    • PEBP1_HUMAN antibody
    • Phosphatidylethanolamine binding protein antibody
    • Phosphatidylethanolamine binding protein 1 antibody
    • Prostatic binding protein antibody
    • Prostatic-binding protein antibody
    • R kip antibody
    • Raf kinase inhibitor protein antibody
    • Raf kinase inhibitory protein antibody
    • RKIP antibody
    see all

Anti-PBP antibody [EPR2875Y] 图像

  • Staining of formalin-fixed, paraffin-embedded human brain tissue using 1/250 ab76582.
  • All lanes : Anti-PBP antibody [EPR2875Y] (ab76582) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling PBP (red) with ab76582 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Anti-PBP antibody [EPR2875Y] (ab76582) at 1/5000 dilution + Recombinant Human PBP protein (ab87892) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa


    Exposure time : 30 seconds

Anti-PBP antibody [EPR2875Y] (ab76582)参考文献

This product has been referenced in:
  • Su L  et al. Raf kinase inhibitor protein regulates oxygen-glucose deprivation-induced PC12 cells apoptosis through the NF-?B and ERK pathways. J Clin Biochem Nutr 59:86-92 (2016). WB, IP . Read more (PubMed: 27698534) »
  • Al-Mulla F  et al. Clinical implications for loss or diminution of expression of Raf-1 kinase inhibitory protein and its phosphorylated form in ductal breast cancer. Am J Cancer Res 3:446-64 (2013). WB, IHC-P ; Human . Read more (PubMed: 24224123) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (neuronal)
Specification
neuronal
Fixative
Formaldehyde
Permeabilization
Yes - a. PBS + 0.2% Triton x-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Aug 31 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (NEURONAL)
Specification
NEURONAL
Fixative
Formaldehyde
Permeabilization
Yes - a. PBS + 0.2% Triton x-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Aug 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal)
Loading amount
50 µg
Specification
neuronal
Gel Running Conditions
Non-reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Aug 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (neuronal)
Loading amount
50 µg
Specification
neuronal
Gel Running Conditions
Non-reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Aug 30 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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