The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 21 kDa.
Use a concentration of 1.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
Belongs to the phosphatidylethanolamine-binding protein family.
ab115682, at 1.5µg/ml, staining PBP in Formalin-fixed, Paraffin-embedded Human brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab115682, at 1.5µg/ml, staining PBP in Formalin-fixed, Paraffin-embedded Human Prostate tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-PBP antibody (ab115682)
Anti-PBP antibody (ab115682) at 0.01 µg/ml + A549 cell lysate (in RIPA buffer) at 35 µg Developed using the ECL technique