RabMAb

Anti-Paxillin (phospho Y181)抗体[EP41(2)Y] (ab76235)

概述

  • 产品名称Anti-Paxillin (phospho Y181)抗体[EP41(2)Y]
    参阅全部 Paxillin 一抗
  • 描述
    兔单克隆抗体[EP41(2)Y] to Paxillin (phospho Y181)
  • 特异性ab76235 only detects Paxillin phosphorylated on Tyrosine 181.
  • 经测试应用适用于: WB, IP, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phosphopeptide corresponding to residues surrounding Tyrosine 181 of Human Paxillin.

  • 阳性对照
    • HeLa cell lysate treated with pervanadate.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab76235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 64 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • 序列相似性Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • 翻译后修饰Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
  • 细胞定位Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ16691 antibody
    • FLJ23042 antibody
    • Paired box protein Pax 1 antibody
    • PAX 1 antibody
    • PAX1 antibody
    • PAXI_HUMAN antibody
    • Paxillin alpha antibody
    • Paxillin antibody
    • PXN antibody
    • PXN protein antibody
    see all

Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] 图像

  • All lanes : Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (ab76235) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 64 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (ab76235)参考文献

This product has been referenced in:
  • Yanagishita T  et al. Actin-binding protein, Espin: a novel metastatic regulator for melanoma. Mol Cancer Res 12:440-6 (2014). WB ; Mouse . Read more (PubMed: 24337068) »
  • Kim SJ  et al. Galectin-3 facilitates cell motility in gastric cancer by up-regulating protease-activated receptor-1 (PAR-1) and matrix metalloproteinase-1 (MMP-1). PLoS One 6:e25103 (2011). WB ; Human . Read more (PubMed: 21966428) »

See all 2 Publications for this product

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