兔单克隆抗体[EP41(2)Y] to Paxillin (phospho Y181)
ab76235 only detects Paxillin phosphorylated on Tyrosine 181.
Synthetic phosphopeptide corresponding to residues surrounding Tyrosine 181 of Human Paxillin.
HeLa cell lysate treated with pervanadate.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 64 kDa).
1/10 - 1/100.
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
Information by UniProt
Paired box protein Pax 1 antibody
Western blot - Paxillin (phospho Y181) antibody [EP41(2)Y] (ab76235)
All lanes :
Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (ab76235) at 1/10000 dilution
Lane 1 :
HeLa cell lysate
Lane 2 :
HeLa cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size :
Observed band size :
68 kDa (
why is the actual band size different from the predicted?
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