概述

  • 产品名称Anti-Paxillin (phospho Y118)抗体
    参阅全部 Paxillin 一抗
  • 描述
    兔多克隆抗体to Paxillin (phospho Y118)
  • 特异性ab75740 detects endogenous levels of Paxillin only when phosphorylated at tyrosine 118.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 118 (H-V-YP-S-F).

  • 阳性对照
    • Human breast carcinoma tissue. Extracts from HeLa cells treated with EGF.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab75740 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 功能Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • 序列相似性Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • 翻译后修饰Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
  • 细胞定位Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ16691 antibody
    • FLJ23042 antibody
    • Paired box protein Pax 1 antibody
    • PAX 1 antibody
    • PAX1 antibody
    • PAXI_HUMAN antibody
    • Paxillin alpha antibody
    • Paxillin antibody
    • PXN antibody
    • PXN protein antibody
    see all

Anti-Paxillin (phospho Y118) antibody 图像

  • ab75740 at 1/50 dilution staining Paxillin in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
  • All lanes : Anti-Paxillin (phospho Y118) antibody (ab75740) at 1/500 dilution

    Lane 1 : HeLa cell extract, untreated
    Lane 2 : HeLa cell extract treated with EGF


    Predicted band size : 64 kDa
    Observed band size : 64 kDa

Anti-Paxillin (phospho Y118) antibody (ab75740)参考文献

This product has been referenced in:
  • Adam AP  et al. Src Family Kinases Modulate the Loss of Endothelial Barrier Function in Response to TNF-a: Crosstalk with p38 Signaling. PLoS One 11:e0161975 (2016). Read more (PubMed: 27603666) »

See 1 Publication for this product

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