概述

  • 产品名称
    Anti-Paxillin (phospho S126)抗体
    参阅全部 Paxillin 一抗
  • 描述
    兔多克隆抗体to Paxillin (phospho S126)
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken
  • 免疫原

    Chemically synthesized phosphopeptide derived from the region of human paxillin that contains serine 126.

  • 阳性对照
    • RAW 264.7 cells treated with LPS.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 1mg/ml BSA(IgG, protease free). pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at serine 126.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab24402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 68 kDa.

靶标

图片

  • All lanes : Anti-Paxillin (phospho S126) antibody (ab24402) at 1/1000 dilution

    Lane 1 : Lysate from untreated RAW 264.7 cells
    Lane 2 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes
    Lane 3 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with non-phosphorylated peptide corresponding to the immunogen
    Lane 4 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with generic phosphoserine-containing peptide
    Lane 5 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with phosphopeptide corresponding to Paxillin [pS91 ]
    Lane 6 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with phosphopeptide immunogen

    Secondary
    All lanes : Goat F(ab)2 anti-rabbit IgG HRP conjugate.

    Observed band size: 68 kDa (why is the actual band size different from the predicted?)



    Lysates were resolved on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% milk-TBST buffer for one hour at room temperature, and then incubated with the Paxillin [pS126] antibody for two hours at room temperature in a 1% milk-TBST buffer, following its prior incubation with or without peptides. Following incubation with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that only the phosphopeptide corresponding to Paxillin [pS 126 ] blocks the antibody signal, thereby demonstrating the specificity of the antibody. No competition was seen following incubation with paxillin phosphopeptides to S130, S178, S380, S455, or S479 (not shown). The antibody was also shown to be specific using 293 cells transfected with wild-type EGFP-tagged human paxillin treated with EGF (not shown).

实验方案

文献

This product has been referenced in:
  • Boeckeler K  et al. Manipulating signal delivery - plasma-membrane ERK activation in aPKC-dependent migration. J Cell Sci 123:2725-32 (2010). WB ; Rat . Read more (PubMed: 20647370) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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