概述

  • 产品名称Anti-Paxillin (phospho S126)抗体
    参阅全部 Paxillin 一抗
  • 描述
    兔多克隆抗体to Paxillin (phospho S126)
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken
  • 免疫原

    Chemically synthesized phosphopeptide derived from the region of human paxillin that contains serine 126.

  • 阳性对照
    • RAW 264.7 cells treated with LPS.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 1mg/ml BSA(IgG, protease free). pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at serine 126.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab24402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 68 kDa.

靶标

Anti-Paxillin (phospho S126) antibody 图像

  • All lanes : Anti-Paxillin (phospho S126) antibody (ab24402) at 1/1000 dilution

    Lane 1 : Lysate from untreated RAW 264.7 cells
    Lane 2 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes
    Lane 3 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with non-phosphorylated peptide corresponding to the immunogen
    Lane 4 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with generic phosphoserine-containing peptide
    Lane 5 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with phosphopeptide corresponding to Paxillin [pS91 ]
    Lane 6 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with phosphopeptide immunogen

    Secondary
    Goat F(ab)2 anti-rabbit IgG HRP conjugate.

    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

Anti-Paxillin (phospho S126) antibody (ab24402)参考文献

This product has been referenced in:
  • Boeckeler K  et al. Manipulating signal delivery - plasma-membrane ERK activation in aPKC-dependent migration. J Cell Sci 123:2725-32 (2010). WB ; Rat . Read more (PubMed: 20647370) »

See 1 Publication for this product

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