参阅全部 Paxillin 一抗
- 特异性This antibody detects endogenous levels of total Paxillin protein.
WB, IHC-P, ELISAmore details
A synthesized non-phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 31 (T-P-YP-S-Y).
- SK-OV3 cell extracts and human breast carcinoma tissue
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
- 纯度Immunogen affinity purified
- 纯化说明The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
||Use at an assay dependent concentration.
- 功能Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
- 序列相似性Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
- 翻译后修饰Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
- 细胞定位Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
Information by UniProt
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
Anti-Paxillin antibody 图像
Western blot - Paxillin antibody (ab47469)
All lanes : Anti-Paxillin antibody (ab47469) at 1/500 dilution
Lane 1 : SK-OV3 cell extracts treated with estradiol (0.1µM, 20min)
Lane 2 : SK-OV3 cell extracts treated with estradiol (0.1µM, 20min) with synthetic peptide
Predicted band size : 64 kDa
Observed band size : 64 kDa
Immunohistochemistry (Paraffin-embedded sections) - Paxillin antibody (ab47469)
Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using ab47469 at a 1/50 dilution.
Anti-Paxillin antibody (ab47469)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"