重组Anti-PAX9抗体[7C2] (ab28538)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7C2] to PAX9
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-PAX9抗体[7C2]
参阅全部 PAX9 一抗 -
描述
大鼠单克隆抗体[7C2] to PAX9 -
宿主
Rat -
特异性
Does not cross react with other PAX proteins -
经测试应用
适用于: ICC/IF, WBmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment within Mouse PAX9 aa 250 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- WB: Mouse esophagus, Mouse skeletal muscle tissue. ICC/IF: NIH/3T3 cells.
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常规说明
This product has switched from a hybridoma to recombinant production method on 08th March 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
7C2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28538于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/20.
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WB |
1/1000. Predicted molecular weight: 38 kDa.
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说明 |
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ICC/IF
1/20. |
WB
1/1000. Predicted molecular weight: 38 kDa. |
靶标
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功能
Transcription factor required for normal development of thymus, parathyroid glands, ultimobranchial bodies, teeth, skeletal elements of skull and larynx as well as distal limbs. -
疾病相关
Defects in PAX9 are the cause of tooth agenesis selective type 3 (STHAG3) [MIM:604625]. A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth). -
序列相似性
Contains 1 paired domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 18511 Mouse
- SwissProt: P47242 Mouse
- Unigene: 5035 Mouse
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别名
- Paired box 9 antibody
- Paired box gene 9 antibody
- Paired box homeotic gene 9 antibody
see all
图片
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All lanes : Anti-PAX9 antibody [7C2] (ab28538) at 1/1000 dilution
Lane 1 : Mouse esophagus tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 38 kDa
Exposure time: 70 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling PAX9 with ab28538 at 1/20 (49.7 µg/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining in NIH/3T3 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab28538 被引用在 11 文献中.
- Pinzon-Guzman C et al. Noggin regulates foregut progenitor cell programming, and misexpression leads to esophageal atresia. J Clin Invest 130:4396-4410 (2020). PubMed: 32427591
- Koterazawa Y et al. Retinoic acid receptor ? activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium. J Gastroenterol 55:763-774 (2020). PubMed: 32556644
- Yuan Y et al. Spatiotemporal cellular movement and fate decisions during first pharyngeal arch morphogenesis. Sci Adv 6:N/A (2020). PubMed: 33328221
- Phillips HM et al. Pax9 is required for cardiovascular development and interacts with Tbx1 in the pharyngeal endoderm to control 4th pharyngeal arch artery morphogenesis. Development 146:N/A (2019). PubMed: 31444215
- Bar C et al. Polycomb Repressive Complex 1 Controls Maintenance of Fungiform Papillae by Repressing Sonic Hedgehog Expression. Cell Rep 28:257-266.e5 (2019). PubMed: 31269445