Anti-PAX8抗体[BC12] (ab124445)

概述

  • 产品名称
    Anti-PAX8抗体[BC12]
    参阅全部 PAX8 一抗
  • 描述
    小鼠单克隆抗体[BC12] to PAX8
  • 特异性
    ab124445 does not stain B-cells, and does not recognize epitopes of pancreatic origin and neuroendocrine cells in stomach and colon.
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cat, Dog, Human
  • 免疫原

    Other Immunogen Type corresponding to PAX8.

  • 阳性对照
    • Human normal kidney, renal cell or serous ovarian carcinoma tissues.
  • 常规说明
    Make dilutions in Renoir Red diluent.

性能

应用

Our Abpromise guarantee covers the use of ab124445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

靶标

  • 功能
    Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • 组织特异性
    Expressed in the excretory system, thyroid gland and Wilms tumors.
  • 疾病相关
    Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • 序列相似性
    Contains 1 paired domain.
  • 发展阶段
    In developing excretory system, during thyroid differentiation and in adult thyroid.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all

图片

  • ab124445 at 1/100 dilution staining PAX8 in formalin-fixed paraffin-embedded Human Renal cell carcinoma tissue by Immunohistochemistry.

文献

ab124445 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (786-0, a renal cell carcinoma cell line)
Loading amount
50 µg
Specification
786-0, a renal cell carcinoma cell line
Gel Running Conditions
Reduced Denaturing (4-12% gradient gel)
Blocking step
Odyssey blocking buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Mar 26 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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