概述

  • 产品名称Anti-PAX7抗体[EPR3353]
    参阅全部 PAX7 一抗
  • 描述
    兔单克隆抗体[EPR3353] to PAX7
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human PAX7.

  • 阳性对照
    • 293T, HeLa, Jurkat or HepG2 cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab92317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
Flow Cyt
  • 应用说明Flow Cyt: 1/200.



    WB: 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.

    Is unsuitable for ICC, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Probable transcription factor. May have a role in myogenesis.
    • 疾病相关Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
    • 序列相似性Belongs to the paired homeobox family.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 paired domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ37460 antibody
      • HUP1 antibody
      • OTTHUMP00000002534 antibody
      • Paired box 7 antibody
      • Paired box gene 7 antibody
      • Paired box homeotic gene 7 antibody
      • Paired box protein Pax-7 antibody
      • Paired domain gene 7 antibody
      • Paired domain gene HuP1 antibody
      • Pax7 antibody
      • PAX7 transcriptional factor antibody
      • PAX7/FKHR fusion gene, included antibody
      • PAX7_HUMAN antibody
      • PAX7B antibody
      • RGD1564360 antibody
      • RMS2 antibody
      see all

    Anti-PAX7 antibody [EPR3353] 图像

    • All lanes : Anti-PAX7 antibody [EPR3353] (ab92317) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 57 kDa
    • Flow cytometric analysis of HeLa cells using ab92317 (red) at a 1/200 dilution, and rabbit IgG control (green).

    Anti-PAX7 antibody [EPR3353] (ab92317)参考文献

    This product has been referenced in:
    • Shi H  et al. Improved regenerative myogenesis and muscular dystrophy in mice lacking Mkp5. J Clin Invest 123:2064-77 (2013). Flow Cyt ; Mouse . Read more (PubMed: 23543058) »

    See 1 Publication for this product

    Product Wall

    Thank you for your inquiry.

    We permeabilized the HeLa cells with 1X FACS permeabilizing solution for 10 minutes and blocked with 0.5% BSA in 1X PBS for 30 minutes. We used a 1:200 primary dilution (30 min at room temperature). We use a FITC ...

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    Gracias por tu respuesta.

    Si estas interesada en usar los dos anticuerpos propuestos para citometría, ten en cuenta que el anti-Myf5 no se ha testado en esta técnica, y por tanto no podemos asegurar que baya a funcionar. Por eso...

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    Gracias por contactarnos.



    He llevado a cabo una búsqueda en nuestro catálogo de los anticuerpos solicitados. Contra PAX7 tenemos un anticuerpo, creado en conejo y monoclonal, que se ha testado en ratones por Citometr&i...

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    Thank you for contacting Abcam yesterday.

    The datasheet for the rabbit isotype control used with this antibody in flow cytometry is attached, along with the protocol that was used.

    Please let me know if there is anything else I can ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"