概述

  • 产品名称Anti-PAX7抗体
    参阅全部 PAX7 一抗
  • 描述
    小鼠单克隆抗体to PAX7
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human, Recombinant Fragment
  • 免疫原

    Recombinant fragment, corresponding to amino acids 411-520 of human PAX7(NP_002575) with a 26 kDa tag.

  • 阳性对照
    • Hela cell lysate; PC-12 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab77180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 57 kDa.

靶标

  • 功能Probable transcription factor. May have a role in myogenesis.
  • 疾病相关Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
  • 序列相似性Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ37460 antibody
    • HUP1 antibody
    • OTTHUMP00000002534 antibody
    • Paired box 7 antibody
    • Paired box gene 7 antibody
    • Paired box homeotic gene 7 antibody
    • Paired box protein Pax-7 antibody
    • Paired domain gene 7 antibody
    • Paired domain gene HuP1 antibody
    • Pax7 antibody
    • PAX7 transcriptional factor antibody
    • PAX7/FKHR fusion gene, included antibody
    • PAX7_HUMAN antibody
    • PAX7B antibody
    • RGD1564360 antibody
    • RMS2 antibody
    see all

Anti-PAX7 antibody 图像

  • Anti-PAX7 antibody (ab77180) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 57 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Anti-PAX7 antibody (ab77180) at 5 µg/ml + Hela cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • Anti-PAX7 antibody (ab77180) at 5 µg/ml + PC-12 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • Detection limit for recombinant tagged PAX7 is approximately 3ng/ml as a capture antibody.

Anti-PAX7 antibody (ab77180)参考文献

ab77180 has not yet been referenced specifically in any publications.

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