概述

  • 产品名称
  • 描述
    兔多克隆抗体to PAX7
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human PAX7.

    (Peptide available as ab37576.)

  • 阳性对照
    • ab34360 was positive in the following Whole Cell Lysates: TE671 (Human Rhabdomyosarcoma cell line).

性能

应用

Our Abpromise guarantee covers the use of ab34360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 20723755
IHC-Fr Use at an assay dependent concentration. PubMed: 22253929

靶标

  • 功能
    Probable transcription factor. May have a role in myogenesis.
  • 疾病相关
    Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
  • 序列相似性
    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ37460 antibody
    • HUP1 antibody
    • OTTHUMP00000002534 antibody
    • Paired box 7 antibody
    • Paired box gene 7 antibody
    • Paired box homeotic gene 7 antibody
    • Paired box protein Pax-7 antibody
    • Paired domain gene 7 antibody
    • Paired domain gene HuP1 antibody
    • Pax7 antibody
    • PAX7 transcriptional factor antibody
    • PAX7/FKHR fusion gene, included antibody
    • PAX7_HUMAN antibody
    • PAX7B antibody
    • RGD1564360 antibody
    • RMS2 antibody
    see all

图片

  • All lanes : Anti-PAX7 antibody (ab34360) at 1 µg/ml

    Lane 1 : Human Uterus Tissue Lysate (negative control)
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.
    Blocking peptides at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab34360 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

文献

This product has been referenced in:
  • Faralli H  et al. UTX demethylase activity is required for satellite cell-mediated muscle regeneration. J Clin Invest 126:1555-65 (2016). Read more (PubMed: 26999603) »
  • Kim CH  et al. Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes. Int J Nanomedicine 11:2407-15 (2016). Read more (PubMed: 27330287) »

See all 20 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skeletal muscle)
Permeabilization
No
Specification
skeletal muscle
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 21 2017

Thank you for contacting us.

Unfortunately, we do not carry a conjugated antibody for PAX7.

However, we carry a number of easy-to-use conjugation kits. Please see the link for the list of kits we carry:

http://www.abcam.c...

Read More

Thank you for your phone call.

I am going to contact the lab shortly regarding ab47052.

The link to our EasyLink conjugation kits can be found here: http://www.abcam.com/Easylink

For the information regarding the discount ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary mouse cells)
Loading amount
20 µg
Specification
primary mouse cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 19 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (muscle sections)
Specification
muscle sections
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 0.1% in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jun 30 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver (human mouse and rat) and mouse small bowel)
Specification
liver (human mouse and rat) and mouse small bowel
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Sequential (prediluated) peroxidase and protein block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Jun 04 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa (Hu), HEK293 (Hu), NIH-3T3, PC-12)
Loading amount
20 µg
Specification
HeLa (Hu), HEK293 (Hu), NIH-3T3, PC-12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Apr 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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