概述

  • 产品名称Anti-PAX7抗体
    参阅全部 PAX7 一抗
  • 描述
    兔多克隆抗体to PAX7
  • 经测试应用适用于: WB, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human PAX7.

    (Peptide available as ab37576.)

  • 阳性对照
    • ab34360 was positive in the following Whole Cell Lysates: TE671 (Human Rhabdomyosarcoma cell line).

性能

应用

Our Abpromise guarantee covers the use of ab34360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 20723755
IHC-Fr Use at an assay dependent concentration. PubMed: 22253929

靶标

  • 功能Probable transcription factor. May have a role in myogenesis.
  • 疾病相关Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
  • 序列相似性Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ37460 antibody
    • HUP1 antibody
    • OTTHUMP00000002534 antibody
    • Paired box 7 antibody
    • Paired box gene 7 antibody
    • Paired box homeotic gene 7 antibody
    • Paired box protein Pax-7 antibody
    • Paired domain gene 7 antibody
    • Paired domain gene HuP1 antibody
    • Pax7 antibody
    • PAX7 transcriptional factor antibody
    • PAX7/FKHR fusion gene, included antibody
    • PAX7_HUMAN antibody
    • PAX7B antibody
    • RGD1564360 antibody
    • RMS2 antibody
    see all

Anti-PAX7 antibody 图像

  • All lanes : Anti-PAX7 antibody (ab34360) at 1 µg/ml

    Lane 1 : Human Uterus Tissue Lysate (negative control)
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.
    Blocking peptides at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab34360 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

Anti-PAX7 antibody (ab34360)参考文献

This product has been referenced in:
  • MacKrell JG  et al. Molecular targets of androgen signaling that characterize skeletal muscle recovery and regeneration. Nucl Recept Signal 13:e005 (2015). Read more (PubMed: 26457071) »
  • Gigliotti D  et al. Atrophy, inducible satellite cell activation, and possible denervation of supraspinatus muscle in injured human rotator-cuff muscle. Am J Physiol Cell Physiol 309:C383-91 (2015). Read more (PubMed: 26135801) »

See all 12 Publications for this product

Product Wall

Thank you for contacting us.

Unfortunately, we do not carry a conjugated antibody for PAX7.

However, we carry a number of easy-to-use conjugation kits. Please see the link for the list of kits we carry:

http://www.abcam.c...

Read More

Thank you for your phone call.

I am going to contact the lab shortly regarding ab47052.

The link to our EasyLink conjugation kits can be found here: http://www.abcam.com/Easylink

For the information regarding the discount ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (primary mouse cells)
Loading amount 20 µg
Specification primary mouse cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 19 2010

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (muscle sections)
Specification muscle sections
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 0.1% in PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jun 30 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (liver (human mouse and rat) and mouse small bowel)
Specification liver (human mouse and rat) and mouse small bowel
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization No
Blocking step Sequential (prediluated) peroxidase and protein block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Jun 04 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa (Hu), HEK293 (Hu), NIH-3T3, PC-12)
Loading amount 20 µg
Specification HeLa (Hu), HEK293 (Hu), NIH-3T3, PC-12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Apr 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"