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Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human PAX7.
(Peptide available as ab37576.)
Our Abpromise guarantee covers the use of ab34360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20723755|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22253929|
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab34360 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406