概述

  • 产品名称
  • 描述
    兔多克隆抗体to PAX6
  • 特异性
    This antibody does not detect bovine PAX6 by Western blot.
  • 经测试应用
    适用于: Flow Cyt, ICC, IP, ICC/IF, WB, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Cow, Dog, Human, Rhesus monkey
    预测可用于: Chicken, Xenopus laevis, Non human primates, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Mouse PAX6 aa 267-285.
    Sequence:

    REEKLRNQRRQASNTPSHI


    (Peptide available as ab5895)

  • 阳性对照
    • Rat whole eye extracts and ovine retinal extracts.

性能

应用

Our Abpromise guarantee covers the use of ab5790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.
ICC/IF 1/50.
WB 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 50.6 kDa).Can be blocked with PAX6 peptide (ab5895).

This antibody can be blocked with ab5895.

IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 17122106

We recommend optimization of antigen retrieval step using buffers for example Citrate or EDTA with incubations for 5, 10, 15 or 20 minutes in microwave or for 2,3,4 or 5 minutes in pressure cooker.

靶标

  • 功能
    Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
  • 组织特异性
    Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.
  • 疾病相关
    Defects in PAX6 are the cause of aniridia (AN) [MIM:106210]. A congenital, bilateral, panocular disorder characterized by complete absence of the iris or extreme iris hypoplasia. Aniridia is not just an isolated defect in iris development but it is associated with macular and optic nerve hypoplasia, cataract, corneal changes, nystagmus. Visual acuity is generally low but is unrelated to the degree of iris hypoplasia. Glaucoma is a secondary problem causing additional visual loss over time.
    Defects in PAX6 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.
    Defects in PAX6 are a cause of foveal hypoplasia (FOVHYP) [MIM:136520]. Foveal hypoplasia can be isolated or associated with presenile cataract. Inheritance is autosomal dominant.
    Defects in PAX6 are a cause of keratitis hereditary (KERH) [MIM:148190]. An ocular disorder characterized by corneal opacification, recurrent stromal keratitis and vascularization.
    Defects in PAX6 are a cause of coloboma ocular (COLO) [MIM:120200]; also known as uveoretinal coloboma or coloboma of iris, choroid and retina. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Severe colobomatous malformations may cause as much as 10% of the childhood blindness. The clinical presentation of ocular coloboma is variable. Some individuals may present with minimal defects in the anterior iris leaf without other ocular defects. More complex malformations create a combination of iris, uveoretinal and/or optic nerve defects without or with microphthalmia or even anophthalmia.
    Defects in PAX6 are a cause of coloboma of optic nerve (COLON) [MIM:120430].
    Defects in PAX6 are a cause of bilateral optic nerve hypoplasia (BONH) [MIM:165550]; also known as bilateral optic nerve aplasia. A congenital anomaly in which the optic disc appears abnormally small. It may be an isolated finding or part of a spectrum of anatomic and functional abnormalities that includes partial or complete agenesis of the septum pellucidum, other midline brain defects, cerebral anomalies, pituitary dysfunction, and structural abnormalities of the pituitary.
    Defects in PAX6 are a cause of aniridia cerebellar ataxia and mental deficiency (ACAMD) [MIM:206700]; also known as Gillespie syndrome. A rare condition consisting of partial rudimentary iris, cerebellar impairment of the ability to perform coordinated voluntary movements, and mental retardation.
  • 序列相似性
    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
  • 发展阶段
    Expressed in the developing eye and brain.
  • 翻译后修饰
    Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AN 2 antibody
    • AN antibody
    • AN2 antibody
    • Aniridia type II protein antibody
    • D11S812E antibody
    • FVH1 antibody
    • KIAA0552 antibody
    • Leucine zipper putative tumor suppressor 3 antibody
    • LZTS3 antibody
    • MGC17209 antibody
    • MGDA antibody
    • Oculorhombin antibody
    • Paired box 6 antibody
    • Paired box gene 6 (aniridia keratitis) antibody
    • Paired Box Gene 6 antibody
    • Paired box homeotic gene 6 antibody
    • Paired box protein Pax-6 antibody
    • Paired box protein Pax6 antibody
    • PAX 6 antibody
    • PAX6 antibody
    • PAX6_HUMAN antibody
    • ProSAP-interacting protein 1 antibody
    • PROSAPIP1 antibody
    • Sey antibody
    • WAGR antibody
    see all

Anti-PAX6 antibody 图像

  • Immunofluorescent analysis of PAX6 (green) in All-Trans-Retinoic Acid (ATRA) treated (10�M for 24 hours) NCCIT cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody(ab5790) at a dilution of 1:50 for at least 1 hour at room temperature and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.


  • Predicted band size : 50.6 kDa
    Western blot analysis of PAX6 was performed by loading 50ug of various Human, mouse and non-human primate whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a PAX6 polyclonal antibody (ab5790) at a dilution of 1:1000 overnight at 4?C on a rocking platform then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:20000 for at least one hour. Chemiluminescent detection was performed.
  • ab5790 at a 1/20 dilution staining PAX6 (green) in cryosectioned human embryoid bodies by Immunocytochemistry/ Immunofluorescence.
  • Immunoprecipitation of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500�g whole cell lysate with 5�g of PAX6 polyclonal antibody (ab5790) overnight on a rocking platform at 4�C. The immune complexes were captured on 50�l Protein A/G Agarose, washed extensively and eluted with 5X Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel then transferred to a PVDF membraneand blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4�C then washed in TBST and probed with detection reagent at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed.
  • ab5790 at 1/100 dilution staining mouse E12.5 whole brain tissue sections by IHC-Fr. The tissue section was incubated with the antibody for 14 hours. An Alexa-Fluor ® 555 conjuagetd donkey antibody was used as the secondary. The image shows clear staining in appropriate cell types in the developing CNS and shows expression in radial glia of the neocortex (PMID: 11896398).

    See Abreview

  • ab5790 staining PAX6 in differentiated mouse ES cells (D3 line) by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 70% methanol. Samples were incubated with primary antibody (1/100 in FACS buffer: 0.2% BSA; 0.1% sodium azide in PBS) for 1 hour. A Phycoerythrin-conjugated goat polyclonal to rabbit IgG (1/500) was used as secondary antibody. Gating strategy: FSC vs SSC on negative control sample.
    Purple profile - negative control
    Green profile - PAX6

    See Abreview

  • All lanes : Anti-PAX6 antibody (ab5790)

    Lane 1 : rat whole eye extract
    Lane 2 : ovine retinal extract


    Predicted band size : 50.6 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    Shows a Western blot of PAX6 on ovine retinal extract using ab5790.
  • ab5790 staining PAX6 in murine brain cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, blocked using 5% BSA for 1 hour at room temperature and then incubated with ab5790 at a 1/250 dilution for 4 hours at 4°C. The secondary used was a Cy2 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.

    See Abreview

Anti-PAX6 antibody (ab5790)参考文献

This product has been referenced in:
  • Yuan X  et al. A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model. PLoS One 12:e0174074 (2017). IF ; Human . Read more (PubMed: 28441409) »
  • Cho SH  et al. Neonatal disease environment limits the efficacy of retinal transplantation in the LCA8 mouse model. BMC Ophthalmol 16:193 (2016). Read more (PubMed: 27809828) »

See all 21 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Xenopus laevis Tissue sections (Whole larvae head)
Antigen retrieval step
None
Permeabilization
Yes - Triton x-100
Specification
Whole larvae head
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 04 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Xenopus laevis Tissue (Larval Cornea)
Specification
Larval Cornea
Username

Abcam user community

Verified customer

提交于 Aug 04 2015

Application
Western blot
Sample
Xenopus laevis Tissue lysate - whole (Eye)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
5 µg
Specification
Eye
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 04 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10%gel)
Sample
Human Cell lysate - whole cell (Human ESCs)
Specification
Human ESCs
Treatment
Insulin 10ug
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 30 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% Gel)
Sample
Mouse Tissue lysate - whole (mouse cerebellum)
Specification
mouse cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 24 2014

Thank you for providing these details.

I am very sorry that this product has not been performing as expected. Having reviewed the protocol details and images I can see no reason which would explain this incorrect staining. I am very sorry ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Cow Cultured Cells (Corneal progenitor cells)
Specification
Corneal progenitor cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 30 2012

Thank you for that information.
I am sorry for the results you have been observing. I am sorry but currently I am not able to explain why you have not observed the expected band of ˜47 kDa.
As well as seeing the expected bands through testing...

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Thank you for taking time to report the problems you have been experiencing. I am sorry to hear that the anti-PAX6 antibody (ab5790) antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and w...

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DISCOUNT CODE: 100%ABR-00000
Expiration date: 30. November 2012
I am very pleased to hear you would like to accept our offer and test ab5790 in cow. This code will give you the value of ab5790 off a future order before the expiration date. Read More

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