概述

  • 产品名称Anti-PAX4抗体
    参阅全部 PAX4 一抗
  • 描述
    兔多克隆抗体to PAX4
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide: RTIFSPSQAE ALEKEFQRGQ YPDSVARGKL ATATSLPEDT VRVWFSNRRA , corresponding to Internal sequence amino acids 184-233 of Human PAX4

  • 阳性对照
    • Jurkat cell lysate; Human intestinal tissue; Human kidney tissue

性能

应用

Our Abpromise guarantee covers the use of ab42450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/312500.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that binds to a common element in the glucagon, insulin and somatostatin promoters. Competes with PAX6 for this same promoter binding site. Isoform 2 appears to be a dominant negative form antagonizing PAX4 transcriptional activity.
  • 疾病相关Defects in PAX4 are a cause of noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2 or maturity-onset diabetes. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Genetic variations in PAX4 are associated with susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100]. IDDM normally starts in childhood or adolescence and is caused by the body's own immune system which destroys the insulin-producing beta cells in the pancreas. Classical features are polydipsia, polyphagia and polyuria, due to hyperglycemia-induced osmotic diuresis.
    Defects in PAX4 are a cause of susceptibility to diabetes mellitus ketosis-prone (KPD) [MIM:612227]. KPD is an atypical form of diabetes mellitus characterized by an acute initial presentation with severe hyperglycemia and ketosis, as seen in classic type 1 diabetes, but after initiation of insulin therapy, prolonged remission is often possible with cessation of insulin therapy and maintenance of appropriate metabolic control. Metabolic studies show a markedly blunted insulin secretory response to glucose, partially reversible with the improvement of blood glucose control. Variable levels of insulin resistance are observed, especially in obese patients. Pancreatic beta-cell autoimmunity is a rare finding.
    Defects in PAX4 are the cause of maturity-onset diabetes of the young type 9 (MODY9) [MIM:612225]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KPD antibody
    • MGC129960 antibody
    • MODY9 antibody
    • Paired box 4 antibody
    • Paired box gene 4 antibody
    • paired box homeotic gene 4 antibody
    • Paired box protein Pax-4 antibody
    • Paired domain gene 4 antibody
    • Pax4 antibody
    • PAX4_HUMAN antibody
    see all

Anti-PAX4 antibody 图像

  • Ab42450 at 4µg/ml staining human PAX4 in human epithelial cells of intestinal villus by immunohistochemistry, parafin embedded human intestine tissue.
  • Ab42450 at 4µg/ml staining human PAX4 in epithelial cells of renal tubule by immunohistochemistry, parafin embedded human kidney tissue.
  • Anti-PAX4 antibody (ab42450) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 37 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

Anti-PAX4 antibody (ab42450)参考文献

This product has been referenced in:
  • Zhang L  et al. KIT is an Independent Prognostic Marker for Pancreatic Endocrine Tumors: A Finding Derived From Analysis of Islet Cell Differentiation Markers. Am J Surg Pathol : (2009). IHC-P ; Human . Read more (PubMed: 19574886) »

See 1 Publication for this product

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I am sorry that these antibodiesdid not perform as stated on the datasheets. If payment has already been made on the original order and y...

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I would be happy to process a refund for you, since these antibodies are covered under our Abpromise, which is good for 6 months after purchase. To be able to process the refund, could you please provide me with the e...

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What I meant by different targets against the same antibody, is that we sometimes have multiply antibodies that recognize the same target in our catalogue.

However, of the 3 antibodies you are using, the only...

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In regards to ab71559, although the 6 month gurantee window has passed, I will make an exception and send a free of charge replacement. Below are the links to a few other HEs1 antibodies that are covered under our Ab...

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Thank you for your reply and for providing the protocol information.

Based on what you have described, I think that the best way forward in this issue is send free of charge replacements for the antibodies in question. I could either replace ...

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Thank you for contacting Abcam.

I am sorry about the issues you have been having with both ab71559 and ab42450 in IHC-P. These antibodies are covered under our Abpromise for six months and are guaranteed to work in IHC-P on human samples. If ...

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Thank you for contacting Abcam.

I am sorry that you are having some technical issues with a few of our antibodies. These antibodiesare covered under our Abpromise for six months andareguaranteed to work in IHC-P on human samples . If we canno...

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