Anti-PAX4抗体(ab101721)

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概述

性能

应用

Our Abpromise guarantee covers the use of ab101721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
Sandwich ELISA Use a concentration of 1 - 2 µg/ml. Used as reporter to detect recombinant PAX4

靶标

  • 功能Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that binds to a common element in the glucagon, insulin and somatostatin promoters. Competes with PAX6 for this same promoter binding site. Isoform 2 appears to be a dominant negative form antagonizing PAX4 transcriptional activity.
  • 疾病相关Defects in PAX4 are a cause of noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2 or maturity-onset diabetes. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Genetic variations in PAX4 are associated with susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100]. IDDM normally starts in childhood or adolescence and is caused by the body's own immune system which destroys the insulin-producing beta cells in the pancreas. Classical features are polydipsia, polyphagia and polyuria, due to hyperglycemia-induced osmotic diuresis.
    Defects in PAX4 are a cause of susceptibility to diabetes mellitus ketosis-prone (KPD) [MIM:612227]. KPD is an atypical form of diabetes mellitus characterized by an acute initial presentation with severe hyperglycemia and ketosis, as seen in classic type 1 diabetes, but after initiation of insulin therapy, prolonged remission is often possible with cessation of insulin therapy and maintenance of appropriate metabolic control. Metabolic studies show a markedly blunted insulin secretory response to glucose, partially reversible with the improvement of blood glucose control. Variable levels of insulin resistance are observed, especially in obese patients. Pancreatic beta-cell autoimmunity is a rare finding.
    Defects in PAX4 are the cause of maturity-onset diabetes of the young type 9 (MODY9) [MIM:612225]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • KPD antibody
    • MGC129960 antibody
    • MODY9 antibody
    • Paired box 4 antibody
    • Paired box gene 4 antibody
    • paired box homeotic gene 4 antibody
    • Paired box protein Pax-4 antibody
    • Paired domain gene 4 antibody
    • Pax4 antibody
    • PAX4_HUMAN antibody
    see all

Anti-PAX4 antibody 图像

  • Western blot - PAX4 antibody (ab101721)
    Western blot - PAX4 antibody (ab101721)
    Anti-PAX4 antibody (ab101721) at 0.2 µg/ml + Mouse pancreas lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 37 kDa
    Observed band size : ~37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

Anti-PAX4 antibody (ab101721)参考文献

ab101721 has not yet been referenced specifically in any publications.

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Immunocytochemistry abreview for Anti-PAX4 antibody

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Application Immunocytochemistry
Sample Mouse Cultured Cells (3T3 Fibroblasts)
Permeabilization Yes - 0.1% Triton-X
Specification 3T3 Fibroblasts
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative Paraformaldehyde
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提交于 Jun 18 2015

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