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Our Abpromise guarantee covers the use of ab15717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 53 kDa.Can be blocked with Human PAX3 peptide (ab23270).|
|IHC-P||Use a concentration of 3 µg/ml.|
|IP||Use a concentration of 2.5 µg/ml.|
ab15717 (3µg/ml) staining of paraffin embedded Human Oesophagus. The tissue sections subjected to antigen retrieval by microwave in Tris/EDTA buffer pH 9.0. The HRP-staining procedure was used for detection.