This antibody gives a positive signal in the following lysates:
Mouse Fetal lysate - 14 days old tissue lysate
Brain (Mouse) Whole Cell Lysate - normal tissue, 0 days old
Mouse Embryonic Fibroblast 1 Whole Cell Lysate
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Use a concentration of 1 µg/ml.
Pax2 is a transcription factor critically required during the development of the nervous and excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract. Like other products of the Pax gene family, Pax2 encodes a conserved 128 amino acid paired box DNA-binding domain in the N-terminal portion of the molecule.
Function: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS.
Tissue specificity: Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.
ICC/IF image of ab38738 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab38738 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Immunohistochemistry (Frozen sections) - Anti-Pax2 antibody (ab38738)This image is courtesy of an abreview submitted by Dr. Ryan MacDonald (Cambridge University, United Kingdom)
IHC-Fr image of Pax2 staining on zebrafish retina sections using ab38738 (1:100). The sections were paraformaldehyde and permeabilized using Triton-X. Antigen retrieval was performed using Tris-HCl and blocking was perfomed using 5% BSA for 1 hour at 23°C. ab38738 was diluted 1:100 and incubated with the sections for 16 hours at 4°C. The secondary antibody was goat polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).
All lanes : Anti-Pax2 antibody (ab38738) at 1 µg/ml
Lane 1 : Mouse brain tissue lysate - total protein (0 days) (ab7188) Lane 2 : Mouse Embryonic Fibroblast 1 Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 44 kDa Observed band size : 44 kDa Additional bands at : 17 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab38738 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Western blot - Pax2 antibody (ab38738)
All lanes : Anti-Pax2 antibody (ab38738) at 1/250 dilution
Lane 1 : Mouse Fetal lysate - 14 Days old Tissue Lysate Lane 2 : Mouse brain tissue lysate - total protein (0 days) (ab7188) Lane 3 : Mouse Embryonic Fibroblast 1 Whole Cell Lysate Lane 4 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
Treviño LS et al. Gene expression profiling reveals differentially expressed genes in ovarian cancer of the hen: support for oviductal origin? Horm Cancer1:177-86 (2010).
Read more (PubMed: 21761365) »
Hurtado A et al. Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen. Nature456:663-6 (2008).
Read more (PubMed: 19005469) »