This antibody gave a positive signal in Mouse and Rat thymus tissue lysates as well as the following whole cell lysates: Jurkat; Raji; K562.
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Immunohistochemistry (Frozen sections) - Anti-PAX1 antibody (ab95227)This image is courtesy of an anonymous Abreview
ab95227 staining PAX1 in mouse E11.5 embryo tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 2% paraformaldehyde in PBS for 2 hours at 4°C then placed in 30% sucrose overnight at 4°C prior to embedding in OCT using liquid nitrogen. Tissue was blocked with 1X PBS + 1.5% Milk + 1.5% BSA + 0.1% Triton X-100 for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 1 hour at 23°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.