Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Pig
Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (SISRILRNKI GSLAQPGPYE ASKQPPSQPT LPYNHIYQYP YPSPVSPTGA) of Human PAX1 (NP_006183).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
This protein is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo. May play an important role in the normal development of the vertebral column.
Contains 1 paired domain.
Information by UniProt
- HUP48 antibody
- OTTHUMP00000030407 antibody
- Paired box 1 antibody
Western blot - Anti-PAX1 antibody (ab83312)
Anti-PAX1 antibody (ab83312) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
46 kDaObserved band size:
40 kDa (why is the actual band size different from the predicted?
Gel concentration: 12%
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