Anti-Patched / PTCH1抗体(ab51983)

概述

  • 产品名称Anti-Patched / PTCH1抗体
    参阅全部 Patched / PTCH1 一抗
  • 描述
    山羊多克隆抗体to Patched / PTCH1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Patched/ PTCH1 aa 1271-1285 (internal sequence).
    Sequence:

    C-HPESRHHPPSNPRQQ


    Database link: Q13635
    (Peptide available as ab200894)

  • 阳性对照
    • Human brain lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab51983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21533246
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 161 kDa).Can be blocked with Human Patched / PTCH1 peptide (ab200894).

靶标

  • 功能Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.
  • 组织特异性In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.
  • 疾病相关Defects in PTCH1 are probably the cause of basal cell nevus syndrome (BCNS) [MIM:109400]; also known as Gorlin syndrome or Gorlin-Goltz syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas (NBCCS) and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas (BCC), fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 is also mutated in squamous cell carcinoma (SCC). Could also be associated with large body size observed in BCNS patients.
    Defects in PTCH1 are a cause of sporadic basal cell carcinoma (BCC) [MIM:605462].
    Defects in PTCH1 are the cause of holoprosencephaly type 7 (HPE7) [MIM:610828]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.
  • 序列相似性Belongs to the patched family.
    Contains 1 SSD (sterol-sensing) domain.
  • 发展阶段In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud.
  • 翻译后修饰Glycosylation is necessary for SHH binding.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A230106A15Rik antibody
    • BCNS antibody
    • FLJ26746 antibody
    • FLJ42602 antibody
    • Holoprosencephaly 7 antibody
    • HPE7 antibody
    • mes antibody
    • NBCCS antibody
    • OTTHUMP00000021709 antibody
    • OTTHUMP00000021710 antibody
    • Patched antibody
    • Patched (Drosophila) homolog antibody
    • Patched 1 antibody
    • Patched homolog (Drosophila) antibody
    • Patched homolog 1 (Drosophila) antibody
    • Patched homolog 1 antibody
    • Patched protein homolog 1 antibody
    • Protein patched homolog 1 antibody
    • PTC antibody
    • PTC1 antibody
    • PTC1_HUMAN antibody
    • PTCH antibody
    • PTCH protein +12b antibody
    • PTCH protein +4' antibody
    • PTCH protein -10 antibody
    • PTCH protein antibody
    • ptch1 antibody
    • PTCH1 protein antibody
    • PTCH11 antibody
    • Ptch2 antibody
    see all

Anti-Patched / PTCH1 antibody 图像

  • Anti-Patched / PTCH1 antibody (ab51983) at 1 µg/ml + Human Brain lysate in RIPA buffer at 35 µg

    Predicted band size : 161 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Patched / PTCH1 antibody (ab51983)参考文献

This product has been referenced in:
  • Konitsiotis AD  et al. Attenuation of hedgehog acyltransferase-catalyzed sonic Hedgehog palmitoylation causes reduced signaling, proliferation and invasiveness of human carcinoma cells. PLoS One 9:e89899 (2014). WB ; Human . Read more (PubMed: 24608521) »
  • Kagawa H  et al. A novel signaling pathway mediated by the nuclear targeting of C-terminal fragments of Mammalian patched 1. PLoS One 6:e18638 (2011). ICC/IF ; Mouse . Read more (PubMed: 21533246) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (fibroblasts)
Gel Running Conditions Reduced Denaturing (4-15%)
Loading amount 100 µg
Specification fibroblasts
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Dr. Adeline Vulin-Chaffiol

Verified customer

提交于 Apr 24 2015

Merci de nous avoir contacté et de nous avoir fournis ces informations supplémentaires. Je suis très désolée que vous avez des problèmes avec cet anticorps.

J'ai étudie le dossier. Effectivement je pense que vous devriez obtenir un bon signa...

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