Chimpanzee, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 1273 -1323 of Human PASK (Q96RG2).
HeLa, 293T and Jurkat cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8.
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 143 kDa.
Use at 2-10 µg/mg of lysate.
Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 2 PAS (PER-ARNT-SIM) domains. Contains 1 protein kinase domain.
Autophosphorylated on Thr-1161 and Thr-1165.
Information by UniProt
Western blot - Anti-PASK antibody (ab115164)
All lanes : Anti-PASK antibody (ab115164) at 0.4 µg/ml Lane 1 : HeLa Cell lysate at 50 µg Lane 2 : HeLa Cell lysate at 15 µg Lane 3 : 293T Cell lysate at 50 µg Lane 4 : Jurkat Cell lysate at 50 µg Predicted band size: 143 kDa Exposure time: 10 seconds
Immunoprecipitation - Anti-PASK antibody (ab115164)
Immunoprecipitation of PASK (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-PASK antibody that recognizes an upstream epitope; Lane 2-Immunoprecipitation with anti-PASK antibody (ab115164); Lane 3- Immunoprecipitation with IgG control. Anti-PASK antibody (ab115164) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.
has not yet been referenced specifically in any publications.
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