Anti-PASK抗体(ab115164)
Key features and details
- Rabbit polyclonal to PASK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PASK抗体
参阅全部 PASK 一抗 -
描述
兔多克隆抗体to PASK -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 1273 -1323 of Human PASK (Q96RG2).
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阳性对照
- HeLa, 293T and Jurkat cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115164于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 143 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 143 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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组织特异性
Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 2 PAS (PER-ARNT-SIM) domains.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated on Thr-1161 and Thr-1165. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 23178 Human
- Omim: 607505 Human
- SwissProt: Q96RG2 Human
- Unigene: 397891 Human
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别名
- DKFZP434O051 antibody
- DKFZp686P2031 antibody
- hPASK antibody
see all
图片
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All lanes : Anti-PASK antibody (ab115164) at 0.4 µg/ml
Lane 1 : HeLa Cell lysate at 50 µg
Lane 2 : HeLa Cell lysate at 15 µg
Lane 3 : 293T Cell lysate at 50 µg
Lane 4 : Jurkat Cell lysate at 50 µg
Predicted band size: 143 kDa
Exposure time: 10 seconds -
Immunoprecipitation of PASK (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-PASK antibody that recognizes an upstream epitope; Lane 2-Immunoprecipitation with anti-PASK antibody (ab115164); Lane 3- Immunoprecipitation with IgG control. Anti-PASK antibody (ab115164) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab115164 尚未被引用在任何文献中。