Synthetic peptide, corresponding to a region within amino acids 425-475 of Human PASK (NP_055963.2).
HeLa, 293T and Jurkat whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab114988 was affinity purified using an epitope specific to PASK immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 143 kDa.
Use at 2-10 µg/mg of lysate.
Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 2 PAS (PER-ARNT-SIM) domains. Contains 1 protein kinase domain.
Autophosphorylated on Thr-1161 and Thr-1165.
Information by UniProt
Anti-PASK antibody 图像
Western blot - Anti-PASK antibody (ab114988)
All lanes : Anti-PASK antibody (ab114988) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 143 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-PASK antibody (ab114988)
Detection of PASK by Western Blot of Immunprecipitate.
ab114988 at 0.4µg/ml staining PASK in HeLa whole cell lysate immunoprecipitated using ab114988 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 mminutes.
Anti-PASK antibody (ab114988)参考文献
has not yet been referenced specifically in any publications.
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