产品名称 Anti-PASK抗体 参阅全部 PASK 一抗
WB, IP more details
Synthetic peptide, corresponding to a region within amino acids 425-475 of Human PASK (NP_055963.2).
HeLa, 293T and Jurkat whole cell lysates.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium azide Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab114988 was affinity purified using an epitope specific to PASK immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 143 kDa.
Use at 2-10 µg/mg of lysate.
组织特异性 Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta.
序列相似性 Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 2 PAS (PER-ARNT-SIM) domains. Contains 1 protein kinase domain.
翻译后修饰 Autophosphorylated on Thr-1161 and Thr-1165.
Information by UniProt
Anti-PASK antibody 图像
Western blot - Anti-PASK antibody (ab114988)
All lanes : Anti-PASK antibody (ab114988) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg developed using the ECL technique Predicted band size : 143 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-PASK antibody (ab114988)
Detection of PASK by Western Blot of Immunprecipitate.
ab114988 at 0.4µg/ml staining PASK in HeLa whole cell lysate immunoprecipitated using ab114988 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 mminutes.
Anti-PASK antibody (ab114988)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"