Anti-PARVB抗体(ab80691)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to PARVB
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: SDLQEEGKNA INSPMSPALV DVHPEDTQLE ENEERTMIDP TSKEDPKFKE , corresponding to N terminal amino acids 71-120 of Human PARVB

  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab80691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-PARVB antibody (ab80691) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab80691 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Xenopus laevis Tissue lysate - other (Whole Xenopus embryos)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
5 µg
Specification
Whole Xenopus embryos
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 May 05 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册