Anti-Parvalbumin抗体(ab94362)

概述

  • 产品名称Anti-Parvalbumin抗体
    参阅全部 Parvalbumin 一抗
  • 描述
    兔多克隆抗体to Parvalbumin
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Horse, Cow, Human, Pig, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Parvalbumin.

  • 阳性对照
    • This antibody gave a positive signal in both Mouse and Rat Cerebellum tissue lysate and rat brain tissue sections

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab94362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

Anti-Parvalbumin antibody 图像

  • All lanes : Anti-Parvalbumin antibody (ab94362) at 1 µg/ml (Blocked with BSA - 5%)

    Lane 1 : Cerebellum Mouse Tissue Lysate
    Lane 2 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 12 kDa


    Exposure time : 2 minutes
  • All lanes : Anti-Parvalbumin antibody (ab94362) at 1 µg/ml (Blocked with MILK - 5%)

    Lane 1 : Cerebellum Mouse Tissue Lysate
    Lane 2 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 12 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • IHC image of ab94362 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94362, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Parvalbumin antibody (ab94362)参考文献

ab94362 has not yet been referenced specifically in any publications.

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