概述

  • 产品名称
    Anti-Parvalbumin抗体
    参阅全部 Parvalbumin 一抗
  • 描述
    兔多克隆抗体to Parvalbumin
  • 经测试应用
    适用于: IHC-FoFr, IHC-Fr, WB, ELISA, IHC-P, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human, Sea urchin
    预测可用于: Gerbil
  • 免疫原

    Full length native protein (purified) corresponding to Rat Parvalbumin. Purified parvalbumin from rat skeletal muscle.

  • 阳性对照
    • Rat cerebellum.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 3% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 20631843
IHC-Fr 1/2000.
WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 12 kDa. Parvalbumin protein is relatively small and, therefore, it is recommended that electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml.
IP Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/200.

靶标

图片

  • Anti-Parvalbumin antibody (ab11427) + rat cerebellum extract
  • Immunocytochemistry/Immunofluorescence analysis of U251 cells labeling Parvalbumin (green) with ab11427 at 1/200. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin ab11427 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Parvalbumin (green) with ab11427 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of C6 cells labeling Parvalbumin (green) with ab11427 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemcial immunofluorescence analysis of 4% PFA & 0.2% Picric acid fixed rat cordical cells in culture, labelling parvalbumin with ab11427 at a dilution of 1/500 incubated for 12 hours at 4°C in 10mM PBS & 0.03% Triton X diluent blend. The secondary was a Donkey anti-Rabbit polyclonal Alexa Fluor® 488 conjugate at 1/200.

    See Abreview

  • ab11427 staining mouse brain cells by ICC/IF.  Cells were PFA fixed and permeabilized with Triton, prior to blocking with 10% serum for 1 hour at RT.  The primary antibody was used undiluted and incubated with the sample for 18 hours at 4°C.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab11427 staining Parvalbumin in human brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using EDTA pH 8.0 for 20 minutes at 100°C. Samples were then incubated with ab11427 at a 1/1000 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/ rabbit IgG.

    See Abreview

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human cerebellum tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin ab114227 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human cerebellum tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin ab114227 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing Parvalbumin ab11427 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

文献

This product has been referenced in:
  • Subashini C  et al. Wnt5a is a crucial regulator of neurogenesis during cerebellum development. Sci Rep 7:42523 (2017). IHC-P ; Mouse . Read more (PubMed: 28205531) »
  • Murphy S  et al. Proteomic profiling of mdx-4cv serum reveals highly elevated levels of the inflammation-induced plasma marker haptoglobin in muscular dystrophy. Int J Mol Med 39:1357-1370 (2017). Read more (PubMed: 28440464) »

See all 69 Publications for this product

客户评价及客户问答

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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I am sorry that the protocol tips that my colleague discussed with you did not prove to be effective and as we talked about, I am sending ab8891 as a free of charge replacement. You should receive the...

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I would be very happy to help you out. Please see my replies to your questions below:

1 - The Citrate buffer antigen retrieval method, should work fine for you. Some antibodies can be more sensitive t...

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Thank you for your enquiry regarding ab11427. Please refer to the product online datasheet for information regarding this product. All the information available for this product is listed on the on-line datasheet (price, datasheet, immunogen, publicati...

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Thank you for your enquiry. We have not tested the frozen protocol using our anti-parvalbumin antibody. One would have to consider the protein of interest and the epitope damage that could occur during freezing, then you will be able to determine whet...

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Thank you for your enquiry. It may indeed be possible to use antibodies in IHC-Fr (frozen sections) when they are already working in IHC-P. This is likely as the fact they work in IHC-P means they have the capacity to recognise the antigen in its na...

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